Simulated gastrointestinal digestion of cranberry polyphenols under dynamic conditions. Impact on antiadhesive activity against uropathogenic bacteria

• Published in: Food chemistry 2022-01, Vol.368, p.130871-130871, Article 130871
• Main Authors: Tamargo, Alba, Cueva, Carolina, Taladrid, Diego, Khoo, Christina, Moreno-Arribas, M. Victoria, Bartolomé, Begoña, González de Llano, Dolores
• Format: Article
• Language: English
• Subjects: Antiadhesive activity; Cranberry; Gut microbiota; Metabolism; Phenolic metabolites; Polyphenols; Short Chain Fatty Acids (SCFAs); Simgi® model; Urinary Tract Infections (UTIs); Uropathogenic Escherichia coli
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2021.130871

•First simulation of gastrointestinal digestion under dynamic conditions of cranberry polyphenols was carried out.•Cranberry polyphenols degradation led to a diversity of bioaccessible microbial-derived metabolites.•Metabolism of cranberry polyphenols promoted an increase in Enterococcaceae population.•Cranberry metabolism modulated the antiadhesive activity against uropathogenic Escherichia coli. This study is the first dynamic simulation of gastrointestinal digestion of cranberry polyphenols [1 g cranberry extract per day (206.2 mg polyphenols) for 18 days]. Samples from the simulated ascending, transverse, and descending colon of the dynamic gastrointestinal simulator simgi® were analyzed. Results showed that 67% of the total cranberry polyphenols were recovered after simulated gastrointestinal digestion. Specifically, benzoic acids, hydroxycinnamic acids, phenylpropionic acids, phenylacetic acids, and simple phenols were identified. Cranberry feeding modified colonic microbiota composition of Enterococcaceae population significantly. However, increments in microbial-derived short-chain fatty acids, particularly in butyric acid, were observed. Finally, the simgi® effluent during cranberry feeding showed significant antiadhesive activity against uropathogenic Escherichia coli (13.7 ± 1.59 % of inhibition). Understanding the role that gut microbiota plays in cranberry metabolism could help to elucidate its interaction with the human body and explain cranberry protective effects against urinary tract infections.