Cloning of Mn-SOD gene and its mRNA expression difference and antioxidant enzyme activities under hypoxia stress of cobia Rachycentron canadum

• Published in: Molecular biology reports 2021-10, Vol.48 (10), p.6897-6909
• Main Authors: Zhang, Jian-Dong, Li, Hong-Juan, Amenyogbe, Eric, Wang, Wei-Zheng, Huang, Jian-Sheng, Chen, Gang
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; 5' Untranslated regions; Amino Acid Sequence; Animal Anatomy; Animal Biochemistry; Animals; Antioxidants; Antioxidants - metabolism; Base Sequence; Biomedical and Life Sciences; Cardiac muscle; Cell Hypoxia; Cloning, Molecular; Complementary DNA; DNA, Complementary - genetics; Environmental factors; Enzymatic activity; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; Histology; Homeostasis; Hypoxia; Intestine; Life Sciences; Liver; Manganese; Models, Molecular; Morphology; Original Article; Oxidative stress; Oxidative Stress - genetics; Perciformes - genetics; Phylogeny; Polymerase chain reaction; Rachycentron canadum; Reactive oxygen species; RNA, Messenger - genetics; RNA, Messenger - metabolism; Spleen; Stress, Physiological; Superoxide dismutase; Superoxide Dismutase - chemistry; Superoxide Dismutase - genetics
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06692-4

Background Environmental hypoxia affects the survival and development of organisms. It is also an important environmental factor that leads to oxidative damage. Hypoxia is a condition in which tissues are deprived of oxygen; reoxygenation is the phenomenon in which hypoxic tissues are exposed to oxygen. Hypoxia-reoxygenation is vital in pathogenesis, where the production of reactive oxygen species and antioxidant disparity significantly contribute to disease progression, and it is one of the most common physiological stressors in the aquaculture industry. Methods and results In this study, the full length of complementary DNA (cDNA) of the manganese superoxide dismutase (Mn-SOD) gene of healthy cobia Rachycentron canadum was analysed using rapid amplification of cDNA ends. The real-time quantitative Polymerase Chain Reaction was used to measure the expression levels of Mn-SOD mRNAs in various tissues (heart, muscle, brain, liver, kidney, gill, intestine, and spleen). The 2 –ΔΔCT method was used to performed the expression analysis. The experimental data were analysed using SPSS ver. 19.0 ( https://spss.software.informer.com/19.0/ ). P