Effects of rice fermented extracts, "Sake Lees", on the functional activity of odontoblast-like cells (KN-3 cells)

[Abstract] This study was designed to investigate the effects of Sake Lees extracts (SLE, Sake Kasu) on the functional activity of odontoblastic cells and tooth pulp of the rats. For in vitro studies, a rat clonal odontoblast-like cell line, KN-3 cells were cultured. SLE significantly decreased KN-3...

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Bibliographic Details
Published in:Odontology 2022-04, Vol.110 (2), p.254-261
Main Authors: Okamoto, Keiichiro, Kakihara, Yoshito, Ohkura, Naoto, Tohma, Aiko, Washio, Ayako, Kitamura, Chiaki, Noiri, Yuichiro, Yamamura, Kensuke, Saeki, Makio
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Animals
Cbfa-1 protein
Cell Differentiation
Cell Line
Cell proliferation
Cytotoxicity
Dental Pulp
Dentin
Dentin sialoprotein
Dentistry
Extracellular Matrix Proteins - metabolism
Fermented food
Medicine
Mineralization
Odontoblasts - drug effects
Odontoblasts - metabolism
Oral and Maxillofacial Surgery
Original Article
Oryza - chemistry
Plant Extracts - pharmacology
Rats
Sake
Teeth
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Summary:[Abstract] This study was designed to investigate the effects of Sake Lees extracts (SLE, Sake Kasu) on the functional activity of odontoblastic cells and tooth pulp of the rats. For in vitro studies, a rat clonal odontoblast-like cell line, KN-3 cells were cultured. SLE significantly decreased KN-3 cell proliferation, but showed no significant cytotoxicity. SLE effects on several protein productions of KN-3 cells were compared with PBS. SLE and PBS increased alkaline phosphatase (ALP), dentin sialoprotein (DSP), and osterix in a day-course dependent manner, while SLE increased the induction of ALP on day 9-21 and DSP on day 15-21. SLE also increased Runx2 expression on day 3 and 9 compared to PBS. Alizarin Red stainings revealed that SLE showed a subtle increase in mineralization of KN-3 cells on day 15 and 21. A histological investigation was conducted to assess if SLE induced reparative dentin formation after direct capping at the exposed tooth pulp in rats, suggesting that SLE could increase the reparative dentin formation more than PBS. These findings suggest that Sake Lees could have functional roles in the alterations of odontoblastic activity, which might influence the physiology of the tooth pulp.
ISSN:1618-1247
1618-1255
DOI:10.1007/s10266-021-00654-9