16 S rRNA gene diversity and gut microbial composition of the Indian white shrimp (Penaeus indicus)

• Published in: Antonie van Leeuwenhoek 2021-12, Vol.114 (12), p.2019-2031
• Main Authors: Patil, Prasanna Kumar, Vinay, Tharabenahalli Nagaraju, Ghate, Sudeep Darbhe, Baskaran, Viswanathan, Avunje, Satheesha
• Format: Article
• Language: English
• Subjects: Abundance; Bacteria; Biomedical and Life Sciences; Composition; Crustaceans; Digestive system; Economic importance; Fenneropenaeus indicus; Gastrointestinal tract; Intestinal microflora; Life Sciences; Medical Microbiology; Microbiology; Microbiota; Microorganisms; Nutrition; Original Paper; Penaeus indicus; Photobacterium; Plant Sciences; Relative abundance; rRNA; Soil Science & Conservation; Vibrio
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01658-9

The endemic Indian white shrimp ( Penaeus indicus ) is an economically important crustacean species, distributed in the Indo-West Pacific region. Knowledge of its gut microbial composition helps in dietary interventions to ensure improved health and production. Here we analyzed V3-V4 hypervariable regions of the 16 S rRNA gene to examine intestinal microbiota in wild and domesticated farmed P. indicus. The study revealed that Proteobacteria, Fusobacteria, Tenericutes , and Bacteroidetes , were the dominant phyla in both the groups although there were differences in relative abundance. The dominant genera in case of the wild group were Photobacterium (29.5 %) followed by Propionigenium (13.9 %), Hypnocyclicus (13.7 %) and Vibrio (11.1 %); while Vibrio (46.5 %), Catenococcus (14 %), Propionigenium (10.3 %) and Photobacterium (8.7 %) were dominant in the farmed group. The results of the study suggest the role of environment on the relative abundance of gut bacteria. This is the first report characterizing gut microbial diversity in P. indicus , which can be used to understand the role of gut microbiota in health, nutrition, reproduction, and growth.