Isolation, characterization, and comparative genomic analysis of vB_PlaP_SV21, new bacteriophage of Paenibacillus larvae

• Published in: Virus research 2021-11, Vol.305, p.198571-198571, Article 198571
• Main Authors: Bozdeveci, Arif, Akpınar, Rahşan, Karaoğlu, Şengül Alpay
• Format: Article
• Language: English
• Subjects: Animals; Bacteriophage; Bacteriophages; Bees - genetics; Genome, Viral; Genomics - methods; Paenibacillus larvae; Paenibacillus larvae - genetics; Paenibacillus phage SV21; Phylogeny; Podoviridae; Podoviridae - genetics
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2021.198571

●The new phage Paenibacillus phage SV21(vB_PlaP_SV21) was isolated, characterized, and compared to other known paenibacilus phages.●The whole-genome analysis determined no known tRNA or antibiotic resistance genes.●Paenibacillus phage SV21(vB_PlaP_SV21) is a new virulent phage belonging to the podoviridae family, which can infect theirs specifically the Paenibacillus larvae causative of AFB disease. Paenibacillus larvae cause an American foulbrood disease (AFB) that is responsible for the extinction of honeybee colonies and is a honeybee bacterial disease that has to be obligatory notified worldwide. Recently, bacteriophage studies targeting Paenibacillus larvae have emerged as a promising alternative treatment method. The inability of bacteria to create resistance against bacteriophages makes this method advantageous. As a consequence, this study was conducted to describe the genome and biological characteristics of a novel phage capable of lysing Paenibacillus larvae samples isolated from honeybee larva samples in Turkey. The Paenibacillus phage SV21 (vB_PlaP_SV21) was isolated by inducing Paenibacillus larvae strain SV21 with Mitomycin-C. Whole-genome sequencing, comparative genomics, and phylogenetic analysis of vB_PlaP_SV21 were performed. Transmission electron microscopy images showed that vB_PlaP_SV21 phage was a Podovirus morphology. The vB_PlaP_SV21 phage specific for Paenibacillus larvae was determined to belong to the Podoviridae family. Host range and specificity, burst size, lytic activity, and morphological characteristics of the phage were determined. Bioinformatic analysis of the Paenibacillus phage SV21 showed 77 coding sequences in its linear 44,949 bp dsDNA genome with a GC content of 39.33%. In this study, we analysed the genomes of all of the currently sequenced P. larvae phage genomes and classified them into five clusters and a singleton. According to molecular, morphological, and bioinformatics results, ıt was observed that API480 (podovirus), which was reported as a singleton in previous studies and public databases, and Paenibacillus phage SV21 phage could form a new cluster together