Antidiabetic effect of konjac glucomannan via insulin signaling pathway regulation in high-fat diet and streptozotocin-induced diabetic rats

• Published in: Food research international 2021-11, Vol.149, p.110664-110664, Article 110664
• Main Authors: Li, Xunhan, Jayachandran, Muthukumaran, Xu, Baojun
• Format: Article
• Language: English
• Subjects: High fat diet; Insulin signaling pathway; Konjac glucomannan; Streptozotocin; Type-2 diabetes mellitus
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2021.110664

[Display omitted] •Treatment with KGM augmented glycolytic enzymes.•KGM diminished gluconeogenesis and liver marker enzymes.•KGM upregulated insulin pathway related gene and protein expression.•KGM positively regulated the glycogen storage. Type 2 diabetes mellitus is a chronic metabolic disorder that tends to disarray various metabolic pathways. Dietary-mediated T2DM prevention garners much attention in recent decades. Hence, this study was intended to elucidate the antidiabetic properties of Konjac glucomannan (KGM) in diabetic rats. Our experimental design includes five groups, with six rats in each group. Group 1 feeding standard diet pallet alone served as control rats; group 2 was KGM control rats administered intragastrically with KGM (120 mg/kg b.w.). Group 3 was developed as diabetic rats with a high-fat diet and an intraperitoneal injection of Streptozotocin-40 mg/kg b.w. Group 4 were diabetic rats treated with KGM (80 mg/kg b.w.), and group 5 were diabetic rats received rosiglitazone treatment (4 mg/kg b.w.). The results showed that STZ-induced diabetic rats significantly elevate liver marker enzymes and gluconeogenesis enzymes. Diminished glycolytic enzymes, liver glycogen, insulin signaling genes, and proteins were also seen in diabetic rats. Treatment with KGM augmented glycolytic enzymes and liver glycogen. On the other hand, KGM diminished gluconeogenesis, liver marker enzymes, upregulated gene, and protein expression of the insulin pathway. The current results suggest dietary KGM can offer a better health benefit in the treatment of T2DM.