The applicability of high‐speed counter‐current chromatography for preparative separation of biosynthesis products: Glycosylation products as example

Biosynthesis is a promising way to manufacture desired products, however, the purification of its final products is a tough work due to the huge amount of reaction matrix. Liquid stationary phase of high‐speed counter‐current chromatography could easily avoid the commonly disadvantages that occurred...

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Bibliographic Details
Published in:Journal of separation science 2021-12, Vol.44 (24), p.4368-4375
Main Authors: Zou, Denglang, Cui, Yunbin, Li, Si, Sang, Duocheng, Liu, Weimeng, Zhao, Tianshu, Gu, Xueli, Chen, Tao, Li, Yulin
Format: Article
Language:English
Subjects:
Acetates - chemistry
Biosynthesis
Chromatography
Column chromatography
Countercurrent Distribution - methods
Ethyl acetate
Glucosides
Glycosylation
Hexanes
Hexanes - chemistry
high‐speed counter‐current chromatography
Ions
Methanol - chemistry
Purification
Separation
Silybin B
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Summary:Biosynthesis is a promising way to manufacture desired products, however, the purification of its final products is a tough work due to the huge amount of reaction matrix. Liquid stationary phase of high‐speed counter‐current chromatography could easily avoid the commonly disadvantages that occurred in traditional column chromatography in the field of biosynthesized products purification. This characteristic makes high‐speed counter‐current chromatography particularly applicable for final products separation in biosynthesis. In this study, the glycosylation products of Silybin B by one‐pot glycosylation were successfully purified by high‐speed counter‐current chromatography to show the applicability of high‐speed counter‐current chromatography for preparative separation of biosynthesis products. An optimized n‐hexane/ethyl acetate/methanol/water (2:5:2:3, v/v/v/v) system was applied in this study. As a result, four Silybin B glycosylation products, including 7 mg of Silybin B‐5‐O‐β‐D‐glucoside (SG‐1), 12 mg of Silybin B‐3‐O‐β‐D‐glucoside (SG‐2), 10 mg of Silybin B‐7‐O‐β‐D‐glucoside (SG‐3), and 24 mg of Silybin B‐20‐O‐β‐D‐glucoside (SG‐4), were simultaneously separated from 200 mg of glycosylation crude products, with the purity of 89.3, 95.2, 96.4, and 97.5%, respectively. Their structures were identified by spectroscopic analysis.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202100544