The regulation of DKGA2ox1 and miR171f_3 in scion dwarfing with Diospyros kaki Thunb. cv. ‘Nan-tong-xiao-fang-shi’ as interstocks

• Published in: Planta 2021-12, Vol.254 (6), p.1-12, Article 113
• Main Authors: Dong, Yuhan, Ye, Xialin, Cao, Lifang, Yu, Xinyi, Qu, Shenchun
• Format: Article
• Language: English
• Subjects: Agriculture; Alcohol dehydrogenase; Biomedical and Life Sciences; Cinnamyl-alcohol dehydrogenase; Complementation; Diospyros - genetics; Diospyros kaki; Ecology; Forestry; Fruit; Gene regulation; Libraries; Life Sciences; MicroRNAs - genetics; miRNA; Next-generation sequencing; ORIGINAL ARTICLE; Persimmons; Plant Growth Regulators; Plant hormones; Plant Sciences; Ribonucleic acid; RNA; Screening; Signal detection; Signal transduction; Transcription Factors; Yeast; Yeasts
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-021-03765-3

Diospyros kaki Thunb. cv. Nan-tong-xiao-fang-shi (‘Nan-tong-xiao-fang-shi’) interstocks play a critical role in the scion dwarfing. However, the understanding of the molecular signaling pathways that regulate the scion dwarfing with ‘Nantong- xiao-fang-shi’ as interstocks remain unclear. In this work, the regulatory network in the scion dwarfing with ‘Nan-tongxiao- fang-shi’ as interstocks was identified. Using a yeast one-hybrid library screening, luciferase activity analysis, luciferase complementation imaging assays and GFP signal detection, a SPL transcription factor named Diospyros kaki SPL (DKSPL), potentially functioning as a transcriptional activator of the Diospyros kaki GA2ox1 (DKGA2ox1) gene, was identified as a key stimulating factor in the persimmon growth and development. The DKSPL was found in the nucleus, and might play a role in the transcriptional regulation system. A microRNA named miR171f_3 was identified, which might act as a negative regulator of Diospyros kaki SCR (DKSCR) in persimmon. The interactions between DKSCR and seven proteins were experimentally validated with a yeast two-hybrid library screening. Compared to the non-grafted wildtype persimmon, the tissue section of graft union healed well due to the increased expression of cinnamyl-alcohol dehydrogenase. These results indicate that DKGA2ox1 and miR171f_3 may co-promote the scion dwarfing by plant hormone signal transduction pathways.