Expression Analysis of AUX/IAA Family Genes in Apple Under Salt Stress

• Published in: Biochemical genetics 2022-08, Vol.60 (4), p.1205-1221
• Main Authors: Li, Yongzhou, Wang, Limin, Yu, Boyang, Guo, Jing, Zhao, Yanan, Zhu, Yuandi
• Format: Article
• Language: English
• Subjects: Abiotic stress; Apples; Auxins; Biochemistry; Biomedical and Life Sciences; Biomedicine; Breeding; Fruits; Genes; Genetic transformation; Genomes; Human Genetics; Hydroponics; Indoleacetic acid; Malus baccata; Medical Microbiology; Original Article; Plant breeding; Plantlets; Polymerase chain reaction; Roots; Salinity tolerance; Salt tolerance; Sodium chloride; Zoology
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-021-10158-4

Members of the auxin/indoleacetic acid (Aux/IAA) gene family in plants are primary auxin-responsive genes that play important roles in many aspects of plant development and in responses to abiotic stress. Recently, 33 Aux/IAA have been identified in the apple genome. The biological responses of MdIAAs to salt stress are still unknown. In this study, Malus zumi , Malus baccata , and Malus  ×  domestica ‘Fuji’ plantlets were subjected to salt stress by supplementing hydroponic media with NaCl at various concentrations. M. zumi showed the strongest salt resistance, followed by ‘Fuji’, and M. baccata was the most sensitive to salt stress. Tissue-specific expression profiles of MdIAAs were determined by quantitative real-time polymerase chain reaction. When apple plantlets were subjected to salt stress, most of salt-responsive MdIAA s were up-regulated by 1 h, 3 h, and 6 h in roots, shoot tips, and leaves, respectively. Highly expressed MdIAAs in roots, especially for M. zumi , consisted with the salt tolerance of apple rootstocks. Transgenic apple calli were tolerant to salt stress when over-expressed salt-responsive genes, MdIAA8 , -9 , and -25 . These results provide clues about salt resistance in these three Malus species, which helps apple breeding of salt tolerance by genetic transformation.