Tracking Stress Granule Dynamics in Live Cells and In Vivo with a Small Molecule

Because of the lack of facile and accurate methods to track stress granule (SG) dynamics in live cells and in vivo, in-depth studies of the biological roles of this attractive membraneless organelle have been limited. Herein, we report the first small-molecule probe, TASG, for the selective, conveni...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-12, Vol.93 (49), p.16297-16301
Main Authors: Shao, Wen, Zeng, Shu-Tang, Yu, Ze-Yi, Tang, Gui-Xue, Chen, Shuo-Bin, Huang, Zhi-Shu, Chen, Xiu-Cai, Tan, Jia-Heng
Format: Article
Language:English
Subjects:
Animals
Biomolecular Condensates
Caenorhabditis elegans
Chemistry
DNA Helicases
Fluorescence
Granular materials
In vivo methods and tests
Poly-ADP-Ribose Binding Proteins
RNA Helicases
RNA Recognition Motif Proteins
Stress
Stress Granules
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Summary:Because of the lack of facile and accurate methods to track stress granule (SG) dynamics in live cells and in vivo, in-depth studies of the biological roles of this attractive membraneless organelle have been limited. Herein, we report the first small-molecule probe, TASG, for the selective, convenient and real-time monitoring of SGs. This novel molecule can simultaneously bind to SG RNAs, the core SG protein G3BP1, and their complexes, triggering a significant enhancement in fluorescence intensity, making TASG broadly applicable to SG imaging under various stress conditions in fixed and live cells, ex vivo and in vivo. Using TASG, the complicated endogenous SG dynamics were revealed in both live cells and C. elegans. Collectively, our work provides an ideal probe that has thus far been absent in the field of SG investigations. We anticipate that this powerful tool may create exciting opportunities to investigate the underlying roles of SGs in different organisms.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c03577