Label‐Free Electrochemiluminescence Imaging of Single‐Cell Adhesions by Using Bipolar Nanoelectrode Array

A label‐free and fast approach for positive electrochemiluminescence (ECL) imaging of single cells by bipolar nanoelectrode array is proposed. The reduction of oxygen at a platinized gold nanoelectrode array in a closed bipolar electrochemical system is coupled with an oxidative ECL process at the a...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry : a European journal 2022-01, Vol.28 (3), p.e202103964-n/a
Main Authors: Qin, Xiang, Jin, Hua‐Jiang, Li, Xiuxiu, Li, Jian, Pan, Jian‐Bin, Wang, Kang, Liu, Songqin, Xu, Jing‐Juan, Xia, Xing‐Hua
Format: Article
Language:English
Subjects:
Adhesion
Adhesive strength
Anodizing
Arrays
Biosensing Techniques
bipolar nanoelectrodes
Cell Adhesion
Cell morphology
Chemistry
Confined spaces
Cytology
Electrochemical Techniques
Electrochemiluminescence
Electrochemistry
Electrodes
Humans
Image acquisition
label-free imaging
Luminescent Measurements
Oxygen
oxygen concentration gradient
Reduction
single cells
Steric hindrance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A label‐free and fast approach for positive electrochemiluminescence (ECL) imaging of single cells by bipolar nanoelectrode array is proposed. The reduction of oxygen at a platinized gold nanoelectrode array in a closed bipolar electrochemical system is coupled with an oxidative ECL process at the anodic side. For elevating the ECL imaging contrast of single cells, a driving voltage of −2.0 V is applied to in situ generate oxygen confined beneath cells that is subsequently used for ECL imaging at 1.1 V. High oxygen concentration in the confined space resulting from steric hindrance generates prominent oxygen reduction current at the cathodic side and higher ECL intensity at the anodic side, allowing positive ECL imaging of the cells adhesion region with excellent contrast. Cell morphology and adhesion strength can be successfully imaged with high image acquisition rate. This approach opens a new avenue for label‐free imaging of single cells. A label‐free method for fast and positive electrochemiluminescence (ECL) imaging of single cells by bipolar nanoelectrode array is proposed using oxygen as the electrochemical probe. The successful imaging is achieved by transient generation of difference in oxygen concentration over the nanoelectrodes covered with or without cells. Thus, cell morphology and adhesion strength can be imaged.
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.202103964