Low-Voltage Bacterial and Viral Killing Using Laser-Induced Graphene-Coated Non-woven Air Filters

• Published in: ACS applied materials & interfaces 2021-12, Vol.13 (49), p.59373-59380
• Main Authors: Gupta, Abhishek, Sharma, Chetan Prakash, Thamaraiselvan, Chidambaram, Pisharody, Lakshmi, Powell, Camilah D, Arnusch, Christopher J
• Format: Article
• Language: English
• Subjects: Air Filters; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Applications of Polymer, Composite, and Coating Materials; Bacteriophage T4 - drug effects; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Graphite - chemistry; Lasers; Materials Testing; Microbial Sensitivity Tests; Particle Size; Pseudomonas aeruginosa - drug effects
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.1c20198

Laser-induced graphene (LIG) is uniquely positioned to advance applications in which electrically conductive carbon coatings are required. Recently, the antifouling, antiviral, and antibacterial properties of LIG have been proven in both air and water filtration applications. For example, an unsupported LIG based filter (pore size: ∼0.3 μm) demonstrated exceptional air filtration properties, while its joule heating effects successfully sterilized and removed unwanted biological components in air despite persisting challenges such as pressure drop, energy consumption, and lack of mechanical robustness. Here, we developed a polyimide (PI) non-woven supported LIG air filter with negligible pressure drop changes compared to the non-woven support material and showed that low electrical current density inactivates aerosolized bacteria. A current density of 4.5 mA/cm2 did not cause significant joule heating, and 97.2% bacterial removal was obtained. The low-voltage antibacterial mechanism was elucidated using bacterial inhibition experiments on a titanium surface and on an LIG surface fabricated on dense PI films. Complete sterilization was obtained using current densities of ∼8 mA/cm2 applied for 2 min or ∼ 6 mA/cm2 for 10 min upon the dense PI–LIG surface. Lastly, >98% bacterial removal was observed using a low-resistance LIG-coated non-woven polyimide air filter at 5 V. However, only very low voltages (∼0.3 V) were needed to remove ∼99% Pseudomonas aeruginosa bacteria and 100% of T4 virus when the LIG-coated filters were hybridized with a stainless steel mesh. Our results show that low current density levels at very low voltages are sufficient for substantial bacterial and viral inactivation, and that these principles might be effectively used in a wide number of air filtration applications such as air conditioners or other ventilation systems, which might limit the spread of infectious particles in hospitals, homes, workplaces, and the transportation industry.