Detachable head-mounted photoacoustic microscope in freely moving mice

Optical resolution photoacoustic microscopy (ORPAM) is a promising tool for investigating anatomical and functional dynamics in the cerebral cortex. However, observation in freely moving mice has been a longstanding challenge for ORPAM. In this Letter, we extended ORPAM from anesthetized, head-restr...

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Bibliographic Details
Published in:Optics letters 2021-12, Vol.46 (24), p.6055-6058
Main Authors: Guo, Heng, Chen, Qian, Qin, Wei, Qi, Weizhi, Xi, Lei
Format: Article
Language:English
Subjects:
Animals
Cerebral cortex
Field of view
Image quality
Laser beams
Mice
Microscopy
Mouse devices
Photoacoustic microscopy
Piezoelectricity
Ultrasonic testing
Ultrasonics
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Summary:Optical resolution photoacoustic microscopy (ORPAM) is a promising tool for investigating anatomical and functional dynamics in the cerebral cortex. However, observation in freely moving mice has been a longstanding challenge for ORPAM. In this Letter, we extended ORPAM from anesthetized, head-restrained to awake, freely moving mice by using a detachable head-mounted ORPAM probe. We used a micro-electro-mechanical-system scanner and a miniaturized piezoelectric ultrasonic detector to scan the excitation laser beam and detect generated photoacoustic signals, respectively. The probe weighs 1.8 g and has a large field of view of ∼3 ×3 . We evaluated the performance of the probe by carrying out phantom experiments and the imaging of vascular networks in a mouse cerebral cortex. The results suggest that the ORPAM probe is capable of providing stable and high-quality ORPAM images in freely moving mice.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.444226