Use of deep sequencing to profile small RNAs derived from tomato spotted wilt orthotospovirus and hippeastrum chlorotic ringspot orthotospovirus in infected Capsicum annuum

• Published in: Virus research 2022-02, Vol.309, p.198648-198648, Article 198648
• Main Authors: Gao, Xue, Jia, Zhi-qiang, Tao, Hong-zheng, Xu, Ye, Li, Yong-zhong, Liu, Ya-ting
• Format: Article
• Language: English
• Subjects: Amaryllidaceae - genetics; Amaryllidaceae - metabolism; Capsicum; Capsicum annuum; High-Throughput Nucleotide Sequencing; Hippeastrum chlorotic ringspot orthotospovirus; Lycopersicon esculentum; Plant defence; Plant Diseases; RNA silencing; RNA Viruses - genetics; RNA, Double-Stranded; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; small RNA sequencing; Tomato spotted wilt orthotospovirus; Tospovirus - genetics; Viral siRNA (vsiRNA)
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2021.198648

•vsiRNA profiles of TSWV-infected and HCRV-infected C. annuum are described.•RNA silencing components have distinct roles in the Orthotospovirus-pepper interaction.•Results provide a basis for further functional characterisation of these genes. Virus-derived small RNAs are one of the key factors of RNA silencing in plant defence against viruses. We obtained virus-derived small interfering RNA profiles from Tomato spotted wilt orthotospovirus and Hippeastrum chlorotic ringspot orthotospovirus infected Capsicum annuum XX19 and XY11 by deep sequencing one day after inoculation. The vsiRNAs data were mapped to the TSWV and HCRV genomes, and the results showed that the vsiRNAs measured 19–24 nucleotides in length. Most of the vsiRNAs were mapped to the S segment of the viral genome. For XX19 and XY11 infected with HCRV, the distribution range of vsiRNAs in S RNA was 52.06–55.20%, while for XX19 and XY11 infected with TSWV, the distribution range of vsiRNAs in S RNA was 87.76–89.07%. The first base at the 5′ end of the siRNA from TSWV and HCRV was primarily biased towards A, U, or C. Compared with mock-inoculated XX19 and XY11, the expression level of CaRDR1 was upregulated in TSWV- and HCRV-inoculated XX19 and XY11. CaAGO2 and CaAGO5 were upregulated in XY11 against HCRV infection, and CaRDR2 was downregulated in TSWV-infected XY11 and XX19. The profile of HCRV and TSWV vsiRNA verified in this study could be useful for selecting key vsiRNA such as those in disease-resistant varieties by artificially synthesizing amiRNA.