Purification and structural characterization of lectin with antibacterial and anticancer properties from grubs of hide beetle, Dermestes frischii

• Published in: International journal of biological macromolecules 2022-04, Vol.203, p.312-332
• Main Authors: Arokiyaraj, Charles, Tamilarasan, Kamalanathan, Manikandan, Ramar, Janarthanan, Sundaram
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Anticancer activity; Bacterial agglutination; Chickens - metabolism; Coleoptera - metabolism; Dermestes frischii; Female; HeLa Cells; Humans; Lectin; Lectins - chemistry
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.01.099

Lectins or haemagglutinins are diverse classes of non-immune proteins; they bind to carbohydrates and are abundant in nature. In the present study, a coleopteran lectin from grubs of hide beetle, Dermestes frischii called DFL, was purified by glutaraldehyde (fixative-agent) fixed hen erythrocytes and characterized further for its functional properties. The purified DFL was stable between pH range 5 to 9 and heat-stable up to 50 °C. It was insensitive to EDTA and did not require any divalent cations. DFL native molecular mass was approximately 69 kDa with three different polypeptide subunits of 33 (pI ~4.4), 22 (pI ~6) and 14 (pI ~4.4) kDa. Haemagglutinating activity of DFL was highly inhibited by N-acetyl-D-glucosamine. DFL partial peptide sequences obtained from peptide mass fingerprinting experiments matched with amino acid sequences of lectins from different organisms confirmed its nature. Biological properties of purified DFL namely antibacterial and bacterial agglutination experiments revealed that DFL have both the effects against laboratory cultures of Aeromonas hydrophila, Enterococcus faecalis, Escherichia coli and habitat bacterial isolates of Staphylococcus cohnii and Bacillus cereus. In addition, the DFL exhibited substantial anticancer properties against HeLa cells. These results concluded that purified DFL could serve as a potent therapeutic agent for various biomedical applications. [Display omitted] •N-acetyl-D-glucosamine specific lectin was purified from grubs of D. frischii.•Purified lectin had a molecular mass of ~69 kDa with three polypeptide subunits.•Lectin possessed both antibacterial and bacterial agglutination properties.•It also exhibited antiproliferative activity against the HeLa cancer cells.