Frequency-Dependent Engagement of Synaptic Vesicle Pools in the Mice Motor Nerve Terminals

Nerve terminals contain numerous synaptic vesicles (SVs) whose exo-endocytic cycling maintains neurotransmitter release. SVs may have different properties, thereby constituting separate pools. However, behavior of SV pools remains elusive in many synapses. To fill this gap, we studied the functionin...

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Bibliographic Details
Published in:Cellular and molecular neurobiology 2023-03, Vol.43 (2), p.729-739
Main Authors: Gafurova, Chulpan R., Tsentsevitsky, Andrei N., Petrov, Alexey M.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cell Biology
Endocytosis
Exocytosis
Frequency dependence
Nerve endings
Neurobiology
Neurosciences
Neurotransmitter release
Original Research
Synaptic vesicles
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Summary:Nerve terminals contain numerous synaptic vesicles (SVs) whose exo-endocytic cycling maintains neurotransmitter release. SVs may have different properties, thereby constituting separate pools. However, behavior of SV pools remains elusive in many synapses. To fill this gap, we studied the functioning of SV pools at both low- and higher-frequency stimulations utilizing microelectrode recording and dual-labeling of SVs with FM-dyes at the mice motor nerve terminals. It was found that higher-frequency stimulation caused exocytosis of different kinds of SVs. One type of SVs contributed to exocytosis exclusively at intense activities and their exocytotic rate was depended on the order in which these SVs were recovered by endocytosis. Another type of SVs can sustain the release in response to both low- and higher-frequency stimulations, but increasing activity did not lead to enhanced exocytotic rate of these SVs. In addition, depression of neurotransmitter release induced by 20 Hz stimulation occurred independent on previous episode of 10 Hz activity. We suggest that during prolonged stimulation at least two SV pools can operate. One termed “house-keeping” that would be active at different frequencies and the other termed “plug-in” that would respond to increasing activity. Graphical Abstract
ISSN:0272-4340
1573-6830
DOI:10.1007/s10571-022-01202-x