Inhibitory effect of d-arabinose on oral bacteria biofilm formation on titanium discs

Biofilm formation on dental implant surfaces can cause peri-implant mucositis and peri-implantitis. Lectins are involved in interactions between bacteria or between bacteria and their hosts. Disrupting these interactions via specific sugars can result in reduced adhesion and biofilm formation. The p...

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Published in:Anaerobe 2022-06, Vol.75, p.102533-102533, Article 102533
Main Authors: An, Sun-Jin, Namkung, Jong-Uk, Ha, Kyung-Won, Jun, Hye-Kyoung, Kim, Hyun Young, Choi, Bong-Kyu
Format: Article
Language:English
Subjects:
Biofilm
d-arabinose
Oral bacteria
Quorum sensing
Titanium discs
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Summary:Biofilm formation on dental implant surfaces can cause peri-implant mucositis and peri-implantitis. Lectins are involved in interactions between bacteria or between bacteria and their hosts. Disrupting these interactions via specific sugars can result in reduced adhesion and biofilm formation. The purpose of this study was to identify sugars that function as antiadhesion or antibiofilm agents on titanium discs. Of the sugars tested, the sugars that did not affect the planktonic growth of Streptococcus oralis, Fusobacterium nucleatum, and Porphyromonas gingivalis were selected. The selected sugars were assessed for their ability to inhibit biofilm formation of bacteria in single and consortium species by crystal violet staining, confocal laser scanning microscopy after live/dead staining, and scanning electron microscopy. The sugars were evaluated for their ability to inhibit activity of the quorum sensing molecule autoinducer 2 (AI-2) by bioluminescence assay. Biofilm formation of single bacteria or consortia of S. oralis, F. nucleatum, and P. gingivalis on titanium discs was significantly inhibited in the presence of d-arabinose. Pretreating titanium discs with d-arabinose for 3 min inhibited biofilm formation at a level comparable to that observed when d-arabinose was present over the entire period, suggesting that d-arabinose had initial anti-adhesive activity. In addition, d-arabinose inhibited the activity of AI-2. d-Arabinose may be a good candidate for application as an antibiofilm agent and AI-2 inhibitor. •d-Arabinose inhibited biofilm formation of S. oralis, F. nucleatum, and P. gingivalis on titanium discs.•Pretreatment of titanium discs with d-arabinose for 3 min resulted in significantly reduced biofilm formation.•d-Arabinose inhibited the activity of autoinducer 2, a quorum sensing molecule.
ISSN:1075-9964
1095-8274
DOI:10.1016/j.anaerobe.2022.102533