Landscape genetics of the tropical willow Salix humboldtiana: influence of climate, salinity, and orography in an altitudinal gradient

• Published in: American journal of botany 2022-03, Vol.109 (3), p.456-469
• Main Authors: Hernández‐Leal, Mariana S., Suárez‐Atilano, Marco, Nicasio‐Arzeta, Sergio, Piñero, Daniel, González‐Rodríguez, Antonio
• Format: Article
• Language: English
• Subjects: Aquatic Organisms; Availability; Chloroplasts; Climate; Colonization; DIGD hypothesis; Ecosystem; Elevation; Flow distribution; Flow rates; Flow velocity; Gene Flow; Genetic distance; Genetic diversity; genetic structure; Genetic Variation; Genetics; Genetics, Population; Hypotheses; Inbreeding; Landscape; Life history; Mexico; Microsatellites; Niches; Optimization; Orography; Population genetics; Population number; Populations; resistance analysis; riparian tree; Rivers; Salicaceae; Salinity; Salinity effects; Salix - genetics; Salix humboldtiana; Willow
• ISSN: 0002-9122; 1537-2197
• DOI: 10.1002/ajb2.1824

Premise Gene flow in riparian ecosystems is influenced by landscape features such as orography, climate, and salinity. The downstream increase in genetic diversity (DIGD) hypothesis states that the unidirectionality of the watercourse causes an accumulation of genetic diversity toward downstream populations, while upstream populations are more structured and less diverse, especially in water‐dispersed organisms. Methods We used chloroplast and nuclear microsatellites to characterize genetic diversity, structure, and gene flow patterns among populations of Salix humboldtiana across an elevation and salinity gradient on three rivers (Actopan, Antigua, and Blanco) in Mexico. We used optimization of resistance surface methods to determine whether genetic distances between populations are correlated with landscape features. Results Positive FIS values evidenced biparental inbreeding in some populations, particularly at higher elevations where lower niche availability constrains colonization and persistence. Four genetic groups were distinguished, corresponding to populations on the Actopan and Antigua rivers and upstream and downstream on the Blanco, but with high admixture between populations on the Actopan and Antigua rivers. Higher gene flow rates were found among proximate populations on the same river than among different rivers. Genetic diversity increased toward the river mouths, in support of the DIGD hypothesis, probably due to greater niche availability and larger population size. Differences among rivers in precipitation patterns and salinity, as well as geographic distance, were significant predictors of gene flow. Conclusions Our results depict that the DIGD and gene flow patterns in S. humboldtiana result from the complex interaction among physiography, climate, river salinity, and life‐history traits of the species.