The combined effects of exercise-induced muscle damage and heat stress on acute kidney stress and heat strain during subsequent endurance exercise

• Published in: European journal of applied physiology 2022-05, Vol.122 (5), p.1239-1248
• Main Authors: Li, Zidong, McKenna, Zachary, Fennel, Zachary, Nava, Roberto Carlos, Wells, Andrew, Ducharme, Jeremy, Houck, Jonathan, Morana, Kylie, Mermier, Christine, Kuennen, Matthew, Magalhaes, Flavio de Castro, Amorim, Fabiano
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Creatine; Creatine kinase; Gelatinase; Heat stress; Heat Stress Disorders; Heat-Shock Response; Human Physiology; Humans; Kidney; Kidneys; Leukocytes (neutrophilic); Lipocalin; Lipocalin-2; Male; Muscle contraction; Muscle, Skeletal - physiology; Occupational Medicine/Industrial Medicine; Original Article; Physical training; Quadriceps muscle; Running; Sensation; Sports Medicine; Temperature
• ISSN: 1439-6319; 1439-6327
• DOI: 10.1007/s00421-022-04919-1

Purpose The purpose of the study was to investigate the combined effect of downhill running and heat stress on muscle damage, as well as on heat strain and kidney stress during subsequent running in the heat. Methods In a randomized cross-over study, ten non-heat-acclimated, physically active males completed downhill running in temperate (EIMD in Temp) and hot (EIMD in Hot) conditions followed by an exercise-heat stress (HS) test after 3-h seated rest. Blood and urine samples were collected immediately pre- and post-EIMD and HS, and 24 h post-EIMD (post-24 h). Core temperature and thermal sensation were measured to evaluate heat strain. Serum creatine kinase (CK), maximal voluntary isometric contraction of the quadriceps (MVC) and perceived muscle soreness were measured to evaluate muscle damage. Urinary neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) levels were measured to indicate acute kidney stress. Results CK, MVC and perceived soreness were not different between conditions at any timepoints. In the EIMD in Hot condition, urinary NGAL was significantly elevated from pre- to post-HS (pre-HS: 6.56 {1.53–12.24} ng/min, post-HS: 13.72 {7.67–21.46} ng/min, p  = 0.034). Such elevation of NGAL or KIM-1 was not found in the EIMD in Temp condition. Conclusions As compared with downhill running in a temperate environment, downhill running in a hot environment does not appear to aggravate muscle damage. However, elevated NGAL levels following EIMD in a hot environment suggest such exercise may increase risk of mild acute kidney injury during subsequent endurance exercise in the heat.