Crystal structure of SFPQ-NONO heterodimer

The Drosophila behavior/human splicing (DBHS) protein family is composed of the three members SFPQ, NONO and PSPC1. These proteins share a strong sequence and structural homology within the core-structured domains forming obligate homo- and heterodimers. This feature may lead to the simultaneous exi...

Full description

Saved in:
Bibliographic Details
Published in:Biochimie 2022-07, Vol.198, p.1-7
Main Authors: Schell, Bianca, Legrand, Pierre, Fribourg, Sébastien
Format: Article
Language:English
Subjects:
DBHS
Heterodimer
Paraspeckle
Structure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Drosophila behavior/human splicing (DBHS) protein family is composed of the three members SFPQ, NONO and PSPC1. These proteins share a strong sequence and structural homology within the core-structured domains forming obligate homo- and heterodimers. This feature may lead to the simultaneous existence of six different dimeric complexes that sustain their function in many cellular processes such as pre-mRNA splicing, innate immunity, transcriptional regulation. In order to perform a complete structural analysis of all possible DBHS dimers, we have solved the crystal structure of the missing DBHS heterodimer SFPQ-NONO at 3.0 Å resolution. We identify subtle changes in amino acid composition and local secondary structure of the NOPS region orientation that may modulate affinity between complexes. Interestingly this area is found mutated in aggressive skin cancers and adenocarcinomas. -Structure of SFPQ-NONO heterodimer-Systematic structural analysis of all DBHS dimers-Identification of potential sites involved in fine-tuning dimer affinity
ISSN:0300-9084
1638-6183
DOI:10.1016/j.biochi.2022.02.011