Advances in chemical and biological characterization of triterpenoid saponins from Anagallis arvensis L. using UHPLC-MS/MS and cell-based assays

A fraction enriched in triterpenoid saponins (F4) from L. was chemically characterized by UHPLC-ESI-QTOF-MS/MS and NMR analyses. The results proposed the presence of nine monodesmosidic saponins derived from oleanolic acid, including two reported for the first time for this species, : 3-O-{β-D-gluco...

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Published in:Natural product research 2023-07, Vol.37 (15), p.2596-2601
Main Authors: Pastoriza, Ana Cristina, Sgariglia, Melina Araceli, Soberón, José Rodolfo, Sampietro, Diego Alejandro
Format: Article
Language:English
Subjects:
Anagallis arvensis
Chromatography, High Pressure Liquid
Cytotoxicity
Humans
Lymphocytes
Molecular Structure
NMR
Nuclear magnetic resonance
Oleanolic acid
Saponins
Saponins - chemistry
Saponins - pharmacology
Tandem Mass Spectrometry
Toxicity testing
Triterpenes - chemistry
Triterpenes - pharmacology
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creator Pastoriza, Ana Cristina
Sgariglia, Melina Araceli
Soberón, José Rodolfo
Sampietro, Diego Alejandro
description A fraction enriched in triterpenoid saponins (F4) from L. was chemically characterized by UHPLC-ESI-QTOF-MS/MS and NMR analyses. The results proposed the presence of nine monodesmosidic saponins derived from oleanolic acid, including two reported for the first time for this species, : 3-O-{β-D-glucopyranosyl (1→4)-[β-D-xylopyranosyl (1→2)]-β-D-glucopyranosyl (1→4)-[β-D-glucopyranosyl (1→2)]-α-L-arabinopyranosyl}-13β, 28-epoxy- 22 acetyl- 28 methoxy-16α, 24-oleananediol and : 3-O-{β-D-glucopyranosyl (1→4)-β-D-glucopyranosyl (1→4)-[β-D-xylopyranosyl (1→2)]-β-D-glucopyranosyl (1→4)-[β-D-glucopyranosyl (1→2)]-α-L-arabinopyranosyl}-13β, 28-epoxy- 16α, 30-oleananediol. Furthermore, haemolytic activity was determined by dot-blot autography, and cytotoxicity on human lymphocyte cultures was analysed according to metabolic activity (MTT assay) and membrane integrity (Trypan blue exclusion test). F4 showed mild cytotoxicity (%V > 50% at 100 μg/mL) on human lymphocytes under conditions of activation or not by LPS; moreover, showed haemolytic activity between 50 and 500 μg. This work contributed to phytochemical knowledge of the triterpenoid saponins from , and its cytotoxic effects on normal human cells.
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subjects Anagallis arvensis
Chromatography, High Pressure Liquid
Cytotoxicity
Humans
Lymphocytes
Molecular Structure
NMR
Nuclear magnetic resonance
Oleanolic acid
Saponins
Saponins - chemistry
Saponins - pharmacology
Tandem Mass Spectrometry
Toxicity testing
Triterpenes - chemistry
Triterpenes - pharmacology
title Advances in chemical and biological characterization of triterpenoid saponins from Anagallis arvensis L. using UHPLC-MS/MS and cell-based assays
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