Maize root exudates recruit Bacillus amyloliquefaciens OR2-30 to inhibit Fusarium graminearum infection

• Published in: Phytopathology 2022-09, Vol.112 (9), p.1886-1893
• Main Authors: Xie, Shanshan, Jiang, Lin, Wu, Qin, Wan, Wenkun, Gan, Yutian, Zhao, Lingling, Wen, Jiajia
• Format: Article
• Language: English
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-01-22-0028-R

Bacillus spp. can exert plant growth-promoting effects and biocontrol effects after effective colonization, and bacterial chemotaxis toward plant root exudates is the initial step to colonize. Under biotic stress, plants are able to alter their root exudates in order to attract or avoid different types of microbes. Hence, Bacillus chemotaxis toward root exudates after pathogen infection is crucial for exerting their beneficial effects. In this study, B. amyloliquefaciens OR2-30 strain, which exhibited greater chemotaxis ability toward maize root exudates after Fusarium graminearum infection, was screened from 156 rhizosphere microorganisms. The infected maize root exudates were further confirmed to improve the swarming and biofilm formation ability of OR2-30 strain. Chemotaxis, swarming and biofilm formation ability were able to influence bacterial colonization. Indeed, the OR2-30 strain displayed more effective colonization ability in maize rhizosphere after F. graminearum inoculation. Moreover, lipopeptides produced by OR2-30 were identified as iturins and responsible for suppressing F. graminearum growth. Further study showed that lipopeptides suppressed the growth of F. graminearum by inhibiting conidia formation and germination, inducing reactive oxygen species (ROS) production and causing cell death in mycelium. Eventually, OR2-30 strain increased maize resistance against F. graminearum. These results suggested that maize root exudates could recruit B. amyloliquefacines OR2-30 after F. graminearum infection, OR2-30 then suppress the F. graminearum by producing lipopeptides, such as iturins, to protect maize.