Accuracy of ASTA MicroIDSys, a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system, for the identification of Korean reference and clinical bacterial and yeast strains

• Published in: Diagnostic microbiology and infectious disease 2022-05, Vol.103 (1), p.115658-115658, Article 115658
• Main Authors: Ko, Young Jin, Lee, O Jin, Lee, Seul-Bi, Kim, Choon-Mee, Lee, Jaehyeon, Kook, Joong-Ki, Park, Soon-Nang, Shin, Jong Hee, Kim, Soo Hyun, Won, Eun Jeong, Park, Geon, Kang, Seong-Ho, Jang, Sook-Jin
• Format: Article
• Language: English
• Subjects: ASTA MicroIDSys system; Bacteria; Bacteria - chemistry; Bruker biotyper system; Humans; Identification; Lasers; MALDI-TOF mass spectrometry; Republic of Korea; Saccharomyces cerevisiae; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Yeast
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2022.115658

This study compared the accuracy of a new MALDI-TOF mass spectrometry system, ASTA MicroIDSys system, with that of MALDI Biotyper system for the identification of reference and clinical bacterial and yeast strains. The identification accuracy of the 2 systems was compared using a total of 406 strains comprising 142 aerobic and 180 anaerobic bacterial strains and 84 yeast strains. The genus and species identification rates were 98.0% and 89.4% using MicroIDSys and 96.1% and 89.4% using Biotyper, respectively. The species identification rates of MicroIDSys and Biotyper for aerobic bacteria were 93.0% and 97.2%, respectively, and those for anaerobic bacteria were 85.6% and 81.7%, respectively. The accuracy of yeast identification at the species level was 91.7% using MicroIDSys and 92.9% using Biotyper. These findings indicate that MicroIDSys could be useful for the accurate identification of bacteria and yeast in clinical microbiology laboratories.