Phytotoxicity Optimization of Fungal Metabolites Produced by Solid and Submerged Fermentation and its Ecotoxicological Effects

• Published in: Applied biochemistry and biotechnology 2022-07, Vol.194 (7), p.2980-3000
• Main Authors: Portela, Valéria Ortaça, Santana, Natielo Almeida, Balbinot, Michele Lusa, Antoniolli, Zaida Inês, de Oliveira Silveira, Andressa, Jacques, Rodrigo Josemar Seminoti
• Format: Article
• Language: English
• Subjects: Bagasse; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Ecological effects; Ecotoxicology; Environmental impact; Fermentation; Fungi; Germination; Glucose; Leaves; Metabolites; Oligochaeta; Optimization; Original Article; Phytotoxicity; Sorghum; Substrates; Sugarcane; Water damage; Worms; Yeasts
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-022-03884-x

Research and commercial production of bioherbicides occur to a lesser extent compared to bioinsecticides and biofungicides. In order to contribute to developing new bioherbicides with low environmental impact, this study aimed to increase the phytotoxicity of metabolites of the fungus Mycoleptodiscus indicus UFSM 54 by optimizing solid and submerged fermentation and evaluate the ecotoxicological effects on earthworms ( Eisenia andrei ). The Plackett–Burman and central composite rotatable designs were used to optimize metabolite phytotoxicity. The variables optimized in the fermentation were temperature, agitation, pH, water volume in the culture medium, glucose concentration, and yeast extract. The fungus was grown on sugarcane bagasse substrate, and its metabolites were applied to detached Cucumis sativus , Conyza sp ., and Sorghum bicolor leaves and used in an avoidance test and acute exposure to earthworms. Metabolite phytotoxicity in submerged fermentation was optimized at 35 °C, 50 rpm, and 1.5 g l −1 of glucose and in solid fermentation at 30–37 °C and in 14–32 ml of water. The metabolites severely damaged germination, initial growth, and leaves of the three plants, and at the doses tested (maximum of 113.92 ml kg −1 ), the metabolites of M. indicus UFSM 54 were not toxic to earthworms.