Detrimental Effect of Trypanosoma brucei brucei Infection on Memory B Cells and Host Ability to Recall Protective B-cell Responses

• Published in: The Journal of infectious diseases 2022-08, Vol.226 (3), p.528-540
• Main Authors: Moon, Sangphil, Janssens, Ibo, Kim, Kyung Hyun, Stijlemans, Benoit, Magez, Stefan, Radwanska, Magdalena
• Format: Article
• Language: English
• Subjects: Antibody response; Antigens; Bone marrow; CD73 antigen; CD80 antigen; Homeostasis; Immunization; Immunoglobulin G; Immunological memory; Lymphocytes B; Memory cells; Parasites; Parasitic diseases; Trypanosoma brucei; Vaccine development; Vaccines; Variant surface glycoprotein
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/jiac112

Abstract Background Trypanosoma brucei brucei evades host immune responses by multiple means, including the disruption of B-cell homeostasis. This hampers anti-trypanosome vaccine development. Because the cellular mechanism underlying this pathology has never been addressed, our study focuses on the fate of memory B cells (MBCs) in vaccinated mice upon trypanosome challenge. Methods A trypanosome variant surface glycoprotein (VSG) and fluorescent phycoerythrin were used as immunization antigens. Functional and cellular characteristics of antigen-specific MBCs were studied after homologous and heterologous parasite challenge. Results Immunization with AnTat1.1 VSG triggers a specific antibody response and isotype-switched CD73+CD273+CD80+ MBCs, delivering 90% sterile protection against a homologous parasite challenge. As expected, AnTat1.1 VSG immunization does not protect against infection with heterologous VSG-switched parasites. After successful curative drug treatment, mice were shown to have completely lost their previously induced protective immunity against the homologous parasites, coinciding with the loss of vaccine-induced MBCs. A phycoerythrin immunization approach confirmed that trypanosome infections cause the general loss of antigen-specific splenic and bone marrow MBCs and a reduction in antigen-specific immunoglobulin G. Conclusions Trypanosomosis induces general immunological memory loss. This benefits the parasites by reducing the stringency for antigenic variation requirements. Using 2 independent vaccination strategies, this study showed that trypanosomosis induces general immunological B-cell memory loss, which benefits the parasite by reducing the stringency for surface antigenic variation.