Stasis and diversity in living fossils: Species delimitation and evolution of lingulid brachiopods

• Published in: Molecular phylogenetics and evolution 2022-10, Vol.175, p.107460-107460, Article 107460
• Main Authors: Goto, Ryutaro, Takano, Tsuyoshi, Seike, Koji, Yamashita, Momo, Paulay, Gustav, Rodgers, Ku'ulei S., Hunter, Cynthia L., Tongkerd, Piyoros, Sato, Shin'ichi, Hong, Jae-Sang, Endo, Kazuyoshi
• Format: Article
• Language: English
• Subjects: Biogeography; Cryptic species; DNA barcoding; Indo-West Pacific; Isthmus of Panama; Lingula
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2022.107460

[Display omitted] •Lingulids are living fossils well-known for stasis in their evolutionary history.•A global molecular phylogeny of the extant Lingulidae was reconstructed.•Lingulids are substantially more species-rich than previously recognized.•Phylogenetic relationships within Lingula and Glottidia were resolved well.•Time-calibrated tree shows Cretaceous origins of the two extant lingulid genera. The Lingulidae are often considered living fossils, because they have shown little morphological change since the Paleozoic. Limited morphological variation has also made the taxonomic study of living lingulids challenging. We investigated species diversity and phylogenetic relationships of extant lingulids and show that they are substantially more diverse than realized, demonstrating that morphological stasis was commonly accompanied by speciation. Species delimitation based on cytochrome c oxidase subunit I (COI) gene sequences from 194 specimens sampled from East Asia, Australia, Oceania, and the Americas suggested 14–22 species in the lingulids (9–17 species in Lingula and 4–5 species in Glottidia), in contrast to the 11–12 species currently recognized globally in the family. Four-gene phylogenetic analyses supported the sister relationship between Lingula and Glottidia. Within Lingula, L. adamsi, which possesses large, brownish shells, was recovered as sister to all remaining Lingula species, which have more or less greenish shells. Within the greenish Lingula clade, the ‘L. anatina’ complex was sister to the clade that includes the ‘L. reevei’ complex. The ‘L. anatina’ complex was further separated into two major clades with partly separate ranges centered on (i) temperate East Asia, and (ii) the tropical west-central Pacific. Within Glottidia, Pacific species were nested within Atlantic species. Time-calibrated phylogenetic analyses suggested that Lingula likely originated in the early Cretaceous contrary to a previously proposed hypothesis advocating a Cenozoic origin. The separation of Lingula and Glottidia appears to date from the Mesozoic, not from the Carboniferous, contrary to a previous hypothesis. Overall, our results uncovered substantial cryptic diversity in lingulids, which will form the basis for conservation and further taxonomic revision.