Untargeted metabolomics and proteomics approach using liquid chromatography-Orbitrap high resolution mass spectrometry to detect pork adulteration in Pangasius hypopthalmus meat

• Published in: Food chemistry 2022-08, Vol.386, p.132856-132856, Article 132856
• Main Authors: Windarsih, Anjar, Suratno, Warmiko, Hendy Dwi, Indrianingsih, Anastasia Wheni, Rohman, Abdul, Ulumuddin, Yaya Ihya
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers; Chromatography, Liquid - methods; Halal authentication; Mass Spectrometry - methods; Meat; Metabolomics - methods; Pangasius hypopthalmus meat; Pork; Pork Meat; Proteomics; Red Meat; Swine; Untargeted metabolomics
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.132856

•Potential metabolite markers of pork and PHM were found using chemometrics.•Pork proteins and metabolites in PHM mixtures could be identified using an untargeted proteomic and metabolomic approach.•This application could be used for other meat-based discovery approaches.•This technique could be used as template methodology for identification of meat adulteration. Pangasius hypopthalmus is well known as a good source of protein. However, Pangasius hypopthalmus meat (PHM) can be adulterated with pork for economic concern, thus, analytical methods for authentication are required. Untargeted metabolomics and proteomics using liquid chromatography-high resolution mass spectrometry (LC-HRMS) and chemometrics of principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) was successfully used to differentiate authentic and adulterated PHM with the good of fitness (R > 0.95) and good of predictivity (Q > 0.5). Metabolites of PC(o-18:0/18:2(9Z,12Z)) was found to be a potential marker for pork whereas DMPC (dimyristoylphosphatidylcholine) was a potential marker for PHM. Meanwhile, pork peptide marker of myoglobin (HPGDFGADAQGAMSK) and β-hemoglobin (FFESFGDLSNADAVMGNPK) could be identified. Both metabolomics and proteomics using LC-HRMS could detect pork at the lowest concentration level (0.5% w/w). In conclusion, untargeted metabolomics and proteomics using LC-HRMS in combination with chemometrics could be used as powerful methods to detect pork adulteration in fish meat.