RBM24 is localized to stress granules in cells under various stress conditions

Stress granules (SGs) are formed when untranslated messenger ribonucleoproteins (mRNPs) accumulate in cells under stress, and are thought to minimize stress-induced damage and promote cell survival. RBM24 (RNA-binding motif protein 24) is an RNA-binding protein that plays pivotal roles in regulating...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2022-06, Vol.608, p.96-101
Main Authors: Wang, Yanfei, Li, Wei, Zhang, Cuiqiao, Peng, Wu, Xu, Zhigang
Format: Article
Language:English
Subjects:
Animals
Cytoplasmic Granules - metabolism
DNA Helicases - metabolism
G3BP1
Knockout
Mice
Poly-ADP-Ribose Binding Proteins - metabolism
RBM24
RNA Helicases - metabolism
RNA interference
RNA Recognition Motif Proteins - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Stress Granules
Stress, Physiological
Zebrafish - metabolism
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Summary:Stress granules (SGs) are formed when untranslated messenger ribonucleoproteins (mRNPs) accumulate in cells under stress, and are thought to minimize stress-induced damage and promote cell survival. RBM24 (RNA-binding motif protein 24) is an RNA-binding protein that plays pivotal roles in regulating the stability or translation initiation of target mRNAs as well as alternative splicing of target pre-mRNAs. Its important physiological functions are highlighted by the fact that Rbm24 knockout mice or zebrafish suffer from dysfunction of heart, eye, and inner ear. Here we show that RBM24 is recruited into SGs under various stress conditions, suggesting that it might protect its target RNAs in cells under stress. However, SG formation is unaffected when Rbm24 expression is down-regulated. Nevertheless, RBM24 overexpression in cultured cells is sufficient to induce SG formation, suggesting that RBM24 might play an important role in SG formation. In conclusion, our present work reveals that RBM24 is a SG component, which implies that RBM24 could protect its target mRNAs in stressed cells. •RBM24 is recruited into SGs under various stress conditions.•SG formation is unaffected when Rbm24 expression is down-regulated.•RBM24 overexpression is sufficient to induce SG formation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2022.03.160