Molecular Detection and Characterization of Sarcocystis Infection in Naturally Infected Buffaloes, Brazil

Sarcocystosis is a disease caused by various Sarcocystis species, a coccidian protozoan parasite that infects humans and animals and is commonly found in ruminants. Although Sarcocystis occurs all over the world, the species responsible for infecting buffaloes in Brazil have not been identified. In...

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Bibliographic Details
Published in:Journal of food protection 2021-03, Vol.84 (3), p.429-428
Main Authors: Portella, Luiza Pires, Fernandes, Fagner D'Ambroso, Minuzzi, Camila EncarnaÇÃo, de Pelegrini, Luis Fernando Vilani, Sangioni, Luis Antonio, Cargnelutti, Juliana Felipetto, Vogel, Fernanda Silveira Flores
Format: Article
Language:English
Subjects:
Animals
Brazil
Brazil - epidemiology
Buffalo
Buffaloes
computer software
Cysts
DNA
Food safety
Genetic testing
Genomic analysis
heart
Infections
Microcysts
Microscopy
nucleotide sequences
Nucleotides
Parasites
Phylogeny
Plastics
Protozoa
Sarcocystis
Sarcocystis - genetics
sarcocystosis
Sarcocystosis - epidemiology
Sarcocystosis - veterinary
Sarcosporidiosis
Sequence analysis
Species
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Summary:Sarcocystosis is a disease caused by various Sarcocystis species, a coccidian protozoan parasite that infects humans and animals and is commonly found in ruminants. Although Sarcocystis occurs all over the world, the species responsible for infecting buffaloes in Brazil have not been identified. In this study, we used molecular methods to estimate the prevalence of natural Sarcocystis infection in buffaloes. Phylogenetic analyses were conducted for the first time to identify the species of this protozoan that are responsible for infecting buffalos in southern Brazil, Rio Grande do Sul state. Heart samples from 80 buffaloes were subjected to microscopic examination followed by molecular analysis. Microcysts were present in 19 (23.75%) of 80 samples. The genomic DNA from the 19 cyst samples was extracted and amplified, and six sequences were obtained. The analysis was performed with the StandenPackage software, and the nucleotide sequences generated were analyzed by comparison with sequences in GenBank. All the sequenced samples were identified as Sarcocystis levinei.
ISSN:0362-028X
1944-9097
DOI:10.4315/JFP-20-130