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Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip‐Powered CRISPR/Cas12a System
The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is profoundly influencing the global healthcare system and people's daily lives. The high resource consumption of coronavirus disease 2019 (COVID‐19) is resulting in insufficient surveillance of coinfection or resurg...
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| Published in: | Small (Weinheim an der Bergstrasse, Germany) Germany), 2022-07, Vol.18 (26), p.e2200854-n/a |
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| container_issue | 26 |
| container_start_page | e2200854 |
| container_title | Small (Weinheim an der Bergstrasse, Germany) |
| container_volume | 18 |
| creator | Liu, Jiajia Wang, Huili Zhang, Li Lu, Ying Wang, Xu Shen, Minjie Li, Nan Feng, Li Jing, Juhui Cao, Bin Zou, Xiaohui Cheng, Jing Xu, Youchun |
| description | The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is profoundly influencing the global healthcare system and people's daily lives. The high resource consumption of coronavirus disease 2019 (COVID‐19) is resulting in insufficient surveillance of coinfection or resurgence of other critical respiratory epidemics, which is of public concern. To facilitate evaluation of the current coinfection situation, a microfluidic system (MAPnavi) is developed for the rapid ( |
| doi_str_mv | 10.1002/smll.202200854 |
| format | article |
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The cocirculation of coronavirus disease 2019 (COVID‐19) with other respiratory diseases, which is associated with a high risk of death, has attracted scant global attention. This study demonstrates a small, rapid (35–40 min), accessible (38–39 °C for all reactions), and automated platform for multiplex virus detection, and a high coinfection rate is found among patients with COVID‐19.</description><identifier>ISSN: 1613-6810</identifier><identifier>EISSN: 1613-6829</identifier><identifier>DOI: 10.1002/smll.202200854</identifier><identifier>PMID: 35599436</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Amplification ; Coronaviruses ; COVID-19 ; CRISPR ; Diagnosis ; Microfluidics ; multiplex diagnosis ; Nanotechnology ; point‐of‐care testing ; respiratory disease ; Respiratory diseases ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Surveillance ; Viral diseases ; Viruses</subject><ispartof>Small (Weinheim an der Bergstrasse, Germany), 2022-07, Vol.18 (26), p.e2200854-n/a</ispartof><rights>2022 Wiley‐VCH GmbH</rights><rights>2022 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3734-78e9fa747723ef2f5aa350a42d9dc38221c7a0e5501bd9b83ee5bf48346026b23</citedby><cites>FETCH-LOGICAL-c3734-78e9fa747723ef2f5aa350a42d9dc38221c7a0e5501bd9b83ee5bf48346026b23</cites><orcidid>0000-0003-4926-8211</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35599436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Jiajia</creatorcontrib><creatorcontrib>Wang, Huili</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Lu, Ying</creatorcontrib><creatorcontrib>Wang, Xu</creatorcontrib><creatorcontrib>Shen, Minjie</creatorcontrib><creatorcontrib>Li, Nan</creatorcontrib><creatorcontrib>Feng, Li</creatorcontrib><creatorcontrib>Jing, Juhui</creatorcontrib><creatorcontrib>Cao, Bin</creatorcontrib><creatorcontrib>Zou, Xiaohui</creatorcontrib><creatorcontrib>Cheng, Jing</creatorcontrib><creatorcontrib>Xu, Youchun</creatorcontrib><title>Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip‐Powered CRISPR/Cas12a System</title><title>Small (Weinheim an der Bergstrasse, Germany)</title><addtitle>Small</addtitle><description>The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is profoundly influencing the global healthcare system and people's daily lives. The high resource consumption of coronavirus disease 2019 (COVID‐19) is resulting in insufficient surveillance of coinfection or resurgence of other critical respiratory epidemics, which is of public concern. To facilitate evaluation of the current coinfection situation, a microfluidic system (MAPnavi) is developed for the rapid (<40 min) and sensitive diagnosis of multiple respiratory viruses from swab samples in a fully sealed and automated manner, in which a nested‐recombinase polymerase amplification and the CRISPR‐based amplification system is first proposed to ensure the sensitivity and specificity. This novel system has a remarkably low limit of detection (50–200 copies mL−1) and is successfully applied to detect 171 clinical samples (98.5% positive predictive agreement; 100% negative predictive agreement), and the results identify 45.6% coinfection among clinical samples from patients with COVID‐19. This approach has the potential to shift diagnostic and surveillance efforts from targeted testing for a high‐priority virus to comprehensive testing of multiple virus sets and to greatly benefit the implementation of decentralized testing.
The cocirculation of coronavirus disease 2019 (COVID‐19) with other respiratory diseases, which is associated with a high risk of death, has attracted scant global attention. This study demonstrates a small, rapid (35–40 min), accessible (38–39 °C for all reactions), and automated platform for multiplex virus detection, and a high coinfection rate is found among patients with COVID‐19.</description><subject>Amplification</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>CRISPR</subject><subject>Diagnosis</subject><subject>Microfluidics</subject><subject>multiplex diagnosis</subject><subject>Nanotechnology</subject><subject>point‐of‐care testing</subject><subject>respiratory disease</subject><subject>Respiratory diseases</subject><subject>Severe acute respiratory syndrome</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Surveillance</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>1613-6810</issn><issn>1613-6829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkU1P20AQhleoFVDaK8dqpV56Sdgvfx0r0wJSUFFSerXW9iwMsr3ujt0oN35CfyO_pI5CU4kLp5nDM49m5mXsVIq5FEKdUds0cyWUEiKNzAE7lrHUszhV2Zt9L8URe0f0IISWyiSH7EhHUZYZHR-z9Qo6wgF_A7ddzZe2x5qfo73rPCFx7_gSqMdgBx82_CeGkXjusXNQDeg7fkvY3XHLr7EK3jUj1ljx_B77p8c_N34NAWqeL69WN8uz3JJUlq82NED7nr11tiH48FxP2O23rz_yy9ni-8VV_mUxq3SizSxJIXM2MUmiNDjlImt1JKxRdVZXOlVKVokVEEVClnVWphogKp1JtYmFikulT9jnnbcP_tcINBQtUgVNYzvwIxUqjreW6TET-ukF-uDH0E3bTVSqYqMTISdqvqOme4kCuKIP2NqwKaQotpEU20iKfSTTwMdn7Vi2UO_xfxlMQLYD1tjA5hVdsbpeLP7L_wKfe5iE</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Liu, Jiajia</creator><creator>Wang, Huili</creator><creator>Zhang, Li</creator><creator>Lu, Ying</creator><creator>Wang, Xu</creator><creator>Shen, Minjie</creator><creator>Li, Nan</creator><creator>Feng, Li</creator><creator>Jing, Juhui</creator><creator>Cao, Bin</creator><creator>Zou, Xiaohui</creator><creator>Cheng, Jing</creator><creator>Xu, Youchun</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4926-8211</orcidid></search><sort><creationdate>20220701</creationdate><title>Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip‐Powered CRISPR/Cas12a System</title><author>Liu, Jiajia ; Wang, Huili ; Zhang, Li ; Lu, Ying ; Wang, Xu ; Shen, Minjie ; Li, Nan ; Feng, Li ; Jing, Juhui ; Cao, Bin ; Zou, Xiaohui ; Cheng, Jing ; Xu, Youchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3734-78e9fa747723ef2f5aa350a42d9dc38221c7a0e5501bd9b83ee5bf48346026b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amplification</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>CRISPR</topic><topic>Diagnosis</topic><topic>Microfluidics</topic><topic>multiplex diagnosis</topic><topic>Nanotechnology</topic><topic>point‐of‐care testing</topic><topic>respiratory disease</topic><topic>Respiratory diseases</topic><topic>Severe acute respiratory syndrome</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Surveillance</topic><topic>Viral diseases</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jiajia</creatorcontrib><creatorcontrib>Wang, Huili</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Lu, Ying</creatorcontrib><creatorcontrib>Wang, Xu</creatorcontrib><creatorcontrib>Shen, Minjie</creatorcontrib><creatorcontrib>Li, Nan</creatorcontrib><creatorcontrib>Feng, Li</creatorcontrib><creatorcontrib>Jing, Juhui</creatorcontrib><creatorcontrib>Cao, Bin</creatorcontrib><creatorcontrib>Zou, Xiaohui</creatorcontrib><creatorcontrib>Cheng, Jing</creatorcontrib><creatorcontrib>Xu, Youchun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jiajia</au><au>Wang, Huili</au><au>Zhang, Li</au><au>Lu, Ying</au><au>Wang, Xu</au><au>Shen, Minjie</au><au>Li, Nan</au><au>Feng, Li</au><au>Jing, Juhui</au><au>Cao, Bin</au><au>Zou, Xiaohui</au><au>Cheng, Jing</au><au>Xu, Youchun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip‐Powered CRISPR/Cas12a System</atitle><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle><addtitle>Small</addtitle><date>2022-07-01</date><risdate>2022</risdate><volume>18</volume><issue>26</issue><spage>e2200854</spage><epage>n/a</epage><pages>e2200854-n/a</pages><issn>1613-6810</issn><eissn>1613-6829</eissn><abstract>The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 is profoundly influencing the global healthcare system and people's daily lives. The high resource consumption of coronavirus disease 2019 (COVID‐19) is resulting in insufficient surveillance of coinfection or resurgence of other critical respiratory epidemics, which is of public concern. To facilitate evaluation of the current coinfection situation, a microfluidic system (MAPnavi) is developed for the rapid (<40 min) and sensitive diagnosis of multiple respiratory viruses from swab samples in a fully sealed and automated manner, in which a nested‐recombinase polymerase amplification and the CRISPR‐based amplification system is first proposed to ensure the sensitivity and specificity. This novel system has a remarkably low limit of detection (50–200 copies mL−1) and is successfully applied to detect 171 clinical samples (98.5% positive predictive agreement; 100% negative predictive agreement), and the results identify 45.6% coinfection among clinical samples from patients with COVID‐19. This approach has the potential to shift diagnostic and surveillance efforts from targeted testing for a high‐priority virus to comprehensive testing of multiple virus sets and to greatly benefit the implementation of decentralized testing.
The cocirculation of coronavirus disease 2019 (COVID‐19) with other respiratory diseases, which is associated with a high risk of death, has attracted scant global attention. This study demonstrates a small, rapid (35–40 min), accessible (38–39 °C for all reactions), and automated platform for multiplex virus detection, and a high coinfection rate is found among patients with COVID‐19.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35599436</pmid><doi>10.1002/smll.202200854</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-4926-8211</orcidid></addata></record> |
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| ispartof | Small (Weinheim an der Bergstrasse, Germany), 2022-07, Vol.18 (26), p.e2200854-n/a |
| issn | 1613-6810 1613-6829 |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Amplification Coronaviruses COVID-19 CRISPR Diagnosis Microfluidics multiplex diagnosis Nanotechnology point‐of‐care testing respiratory disease Respiratory diseases Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Surveillance Viral diseases Viruses |
| title | Sensitive and Rapid Diagnosis of Respiratory Virus Coinfection Using a Microfluidic Chip‐Powered CRISPR/Cas12a System |
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