Secondary metabolites produced by Macrophomina phaseolina, a fungal root endophyte of Brugmansia aurea, using classical and epigenetic manipulation approach

Endophytic fungi are rich sources of structurally complex chemical scaffolds with interesting biological activities. However, their metabolome is still unknown, making them appealing for novel compound discovery. To maximize the number of secondary metabolites produced from a single microbial source...

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Bibliographic Details
Published in:Folia microbiologica 2022-10, Vol.67 (5), p.793-799
Main Authors: Singh, Gurpreet, Kumar, Amit, Verma, M. K., Gupta, Prasoon, Katoch, Meenu
Format: Article
Language:English
Subjects:
Acids
Applied Microbiology
Benzene
Biomedical and Life Sciences
Chemicals
Dextrose
Endophytes
Environmental Engineering/Biotechnology
Epigenetics
Ergosterol
Fungi
Immunology
Integrative medicine
Laboratories
Life Sciences
Metabolites
Microbiology
Microorganisms
Natural products
Original Article
Propanol
Secondary metabolites
Sodium
Sodium valproate
Solvents
Toluene
Valproic acid
Volatile compounds
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Summary:Endophytic fungi are rich sources of structurally complex chemical scaffolds with interesting biological activities. However, their metabolome is still unknown, making them appealing for novel compound discovery. To maximize the number of secondary metabolites produced from a single microbial source, we used the “OSMAC (one strain–many compounds) approach.” In potato dextrose medium, M. phaseolina produced phomeolic acid (1), ergosterol peroxide (2), and a volatile compound 1,4-benzene-diol. Incorporating an epigenetic modifier, sodium valproate, affected the metabolite profile of the fungus. It produced 3-acetyl-3-methyl dihydro-furan-2(3H)-one (3) and methyl-2-(methyl-thio)-butyrate (4), plus volatile chemicals: butylated hydroxy toluene (BHT), di-methyl-formamide, 3-amino-1-propanol, and 1,4-benzenediol, 2-amino-1-(O-methoxyphenyl) propane. The structure of compounds 1–4 was established with the help of spectroscopic data. This study revealed first-time compounds 1–4 in the fungus M. phaseolina  using a classical and epigenetic manipulation approach.
ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-022-00976-3