Molecular characterization of carbapenem-resistant Pseudomonas aeruginosa isolated from four medical centres in Iran

Background Understanding the mechanisms of antibiotic resistance is important for designing new therapeutic options and controlling resistant strains. The goal of this study was to look at the molecular epidemiology and mechanisms of resistance in carbapenem-resistant Pseudomonas aeruginosa (CRPA) i...

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Bibliographic Details
Published in:Molecular biology reports 2022-09, Vol.49 (9), p.8281-8289
Main Authors: Khalili, Younes, Omidnia, Pooya, Goli, Hamid Reza, Zamanlou, Sajjad, Babaie, Farhad, Zahedi Bialvaei, Abed, Esmailkhani, Aylin
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Antibiotic resistance
Antibiotics
Biomedical and Life Sciences
Carbapenemase
Cross-infection
Epidemiology
Genotyping
Histology
Life Sciences
Morphology
Original Article
Polymerase chain reaction
Pseudomonas aeruginosa
Random amplified polymorphic DNA
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Summary:Background Understanding the mechanisms of antibiotic resistance is important for designing new therapeutic options and controlling resistant strains. The goal of this study was to look at the molecular epidemiology and mechanisms of resistance in carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates from Tabriz, Iran. Methods One hundred and forty P. aeruginosa were isolated and antibiotic susceptibility patterns were determined. Overproduction of AmpC and efflux pumps were discovered using phenotypic techniques. Polymerase chain reaction (PCR) was used to determine the presence of carbapenemase-encoding genes. In addition, the expressions of OprD and efflux pumps were evaluated by the Real-Time PCR. Random amplified polymorphic DNA typing (RAPD) was performed for genotyping. Results Among 140 P. aeruginosa isolates, 74 (52.8%) were screened as CRPA. Overexpression of efflux systems was observed in 81% of isolates, followed by decreased expression of OprD (62.2%), presence of carbapenemase genes (14.8%), and overproduction of AmpC (13.5%). In most isolates, carbapenem resistance was multifactorial (60.8%). According to our results, the prevalence of CRPA is at alarming levels. Overexpression of efflux systems was the most common mechanism of carbapenem resistance. Conclusion Most isolates may originate in patients themselves, but cross-infection is possible. Therefore, we suggest a pattern shift in the strategy of CRPA in our setting.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-022-07640-6