Exogenous melatonin enhances soybean (Glycine max (L.) Merr.) seedling tolerance to saline‐alkali stress by regulating antioxidant response and DNA damage repair

Saline‐alkali (SA) stress induces excessive reactive oxygen species (ROS) accumulation in plant cells, resulting in oxidative damages of membranes, lipids, proteins, and nucleic acids. Melatonin has antioxidant protection effects in living organisms and thus has received a lot of attention. This stu...

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Published in:Physiologia plantarum 2022-07, Vol.174 (4), p.e13731-n/a
Main Authors: Zhao, Qiang, Chen, Suyu, Wang, Guangda, Du, Yanli, Zhang, Zhaoning, Yu, Gaobo, Ren, Chunyuan, Zhang, Yuxian, Du, Jidao
Format: Article
Language:English
Subjects:
Accumulation
Alkalies - metabolism
Alkalies - pharmacology
Antioxidants
Antioxidants - metabolism
Apoptosis
Apurinic sites
Cultivars
Damage accumulation
Deoxyribonucleic acid
DNA
DNA Damage
DNA repair
Fabaceae - genetics
G2 Phase Cell Cycle Checkpoints
Glycine max - metabolism
Lipids
Melatonin
Melatonin - pharmacology
Nucleic acids
Oxidative Stress
Plant cells
Polymorphism
Random Amplified Polymorphic DNA Technique
Reactive oxygen species
Reactive Oxygen Species - metabolism
Repair
Roots
Salt Stress
Seedlings
Soybeans
Specific gravity
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Summary:Saline‐alkali (SA) stress induces excessive reactive oxygen species (ROS) accumulation in plant cells, resulting in oxidative damages of membranes, lipids, proteins, and nucleic acids. Melatonin has antioxidant protection effects in living organisms and thus has received a lot of attention. This study aimed to investigate the effect and regulating mechanism of melatonin treatment on soybean tolerance to SA stress. In this study, cultivars Heihe 49 (HH49, SA‐tolerant) and Henong 95 (HN95, SA‐sensitive) were pot‐cultured in SA soil, then treated with MT (0–300 μM) at V1 stage. SA stress induced ROS accumulation and DNA damage in the seedling roots of both cultivars, causing G1/S arrest in HN95 and G2/M arrest in HH49. Melatonin treatment enhanced the activity of antioxidant enzymes in soybean seedling roots and reduced ROS accumulation. Additionally, melatonin treatment upregulated DNA damage repair genes, thus enhancing the reduction of DNA oxidative damage under SA stress. The effects of melatonin treatment were manifested as decreased RAPD polymorphism, 8‐hydroxy‐2′‐deoxyguanine (8‐OH‐dG) level, and relative density of apurinic sites (AP‐sites). Meanwhile, melatonin treatment partially alleviated the SA‐induced G1/S arrest in HN95 and G2/M arrest in HH49, thus enhancing soybean seedling tolerance to SA stress.
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.13731