The noninvasive monitoring of the redox status of photosynthetic electron transport chains in Hibiscus rosa-sinensis and Tradescantia leaves

• Published in: Plant physiology and biochemistry 2022-08, Vol.185, p.233-243
• Main Authors: Suslichenko, Igor S., Trubitsin, Boris V., Vershubskii, Alexey V., Tikhonov, Alexander N.
• Format: Article
• Language: English
• Subjects: Electron paramagnetic resonance; Electron transport; Hibiscus rosa-Sinensis; P700 photooxidation; Photosynthesis; Plastoquinone; Tradescantia
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2022.06.002

We present an approach to the noninvasive determination of the electron capacity of the intersystem pool of electron carriers in chloroplasts in situ. As apt experimental models, we used the leaves of Hibiscus rosa-sinensis and Tradescantia species. Electron paramagnetic resonance and optical response of P700 (the primary electron donor in Photosystem I) were applied to measuring electron transport in chloroplasts. Electron capacities of the intersystem electron transport chain (ETC) were determined from redox transients of P700 upon chromatic transitions (white light → far-red light). During the induction period, we observed the nonmonotonic changes in the number of electron equivalents in the intersystem ETC per P700 (parameter Q). In Hibiscus rosa-sinensis, the light-induced rise of Q from ≈2.5 (in the dark) to Q ≈ 12 was followed by its decrease to Q ≈ 6. The data obtained are discussed in the context of pH-dependent regulation of electron transport in chloroplasts, which provides the well-balanced operation of the intersystem ETC. The decay of Q is explained by the attenuation of Photosystem II activity due to the lumen acidification and the acceleration of plastoquinol re-oxidation as a result of the Calvin-Benson cycle activation. Our computer model of electron and proton transport coupled to ATP synthesis in chloroplasts was used to analyze the up and down feedbacks responsible for pH-dependent regulation of electron transport in chloroplasts. The procedures introduced here may be important for subsequent works aimed at defining the plastoquinone participation in regulation of photosynthetic processes in chloroplasts in situ. •Method for determination of the intersystem electron capacity is developed.•We demonstrated non-monotonic dynamics of plastoquinone reduction/oxidation.•Computer model of electron and proton transport in chloroplasts is developed.