Purification, Characterization, and Application of Endoglucanase from Rhizopus oryzae as Antibiofilm Agent

• Published in: Applied biochemistry and biotechnology 2023-09, Vol.195 (9), p.5439-5457
• Main Authors: Karmakar, Moumita, Lahiri, Dibyajit, Nag, Moupriya, Dutta, Bandita, Dash, Sudipta, Sarkar, Tanmay, Pandit, Soumya, Upadhye, Vijay Jagdish, Ray, Rina Rani
• Format: Article
• Language: English
• Subjects: Bacteria; Biochemistry; biofilm; Biofilms; Biotechnology; Carboxymethyl cellulose; Carboxymethylcellulose; Cellulose; Chemistry; Chemistry and Materials Science; computer simulation; Copper; cross infection; Depolymerization; endo-1,4-beta-glucanase; Endoglucanase; Enzymes; Fungi; Gel chromatography; glucose; Homogeneity; hydrolysis; Molecular docking; Molecular weight; Nosocomial infection; Original Article; Proteins; Purification; Rhizopus oryzae; Substrate preferences; Substrates; temperature; Temperature preferences; Thiols; Ultrafiltration
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-022-04043-y

The enzyme endoglucanase is responsible for the depolymerization of cellulose. This study focuses on characterization and purification of endoglucanase from  Rhizopus oryzae  MTCC 9642 through a simple size exclusion method and its effective application as an antibiofilm agent. Extracellular ß-1,4-endoglucanase, an enzyme that catalyzes the hydrolysis of carboxymethyl cellulose, was found to be synthesized by  Rhizopus oryzae  MTCC 9642. The enzyme was purified up to homogeneity simply by size exclusion process through ultrafiltration and gel chromatography. The molecular weight of purified enzyme protein was estimated to be 39.8 kDa and it showed the highest substrate affinity towards carboxymethyl-cellulose with K m  and V max  values of 0.833 mg ml −1  and of 0.33 mmol glucose min −1  mg −1 protein, respectively. The purified enzyme exhibited optimal activity at pH 6 with a broad stability range of pH 3–8. The most preferred temperature was 35 °C and 50% of activity could be retained after the thermal exposure at 40 °C for 25 min. The purified enzyme protein was inactivated by Cu 2+ , while the activity could be enhanced by the addition of exogenous thiols. Since biofilm is a challenge for health sector, with the aim of eradicating the biofilm, the purified endoglucanase was used to remove biofilm produced by two nosocomial bacteria. As predicted by in silico molecular docking interaction, the purified enzyme could effectively degrade biofilm architecture of bacterial strains S. aureus and P. aeruginosa by 76.52 ± 6.52% and 61.67 ± 8.76%, respectively. The properties of purified enzyme protein, as elucidated by in vitro and in silico characterization, may be favourable for its commercial applications as a potent antibiofilm agent.