Ontogeny of the trigeminal system and associated structures in Alligator mississippiensis

• Published in: Journal of morphology (1931) 2022-09, Vol.283 (9), p.1210-1230
• Main Authors: Lessner, Emily J., Elsey, Ruth M., Holliday, Casey M.
• Format: Article
• Language: English
• Subjects: cranial nerves; epipterygoid; homology; topology
• ISSN: 0362-2525; 1097-4687
• DOI: 10.1002/jmor.21498

From the appearance of the vertebrate head, the trigeminal system has played a role in behavioral and ecological adaptation. The trigeminal nerve is the primary cranial somatosensory nerve, also innervating the jaw muscles. In crocodylians, the trigeminal nerve plays a role in modulating the high bite force and unique integumentary sensation. In association with these behaviors, crocodylians are known for large trigeminal nerves, a high volume of trigeminal‐innervated musculature, and densely packed, specialized sensory receptors. These innovations also occurred in concert with a restructuring of the lateral braincase wall. These morphologies have previously been investigated in phylogenetic and evolutionary contexts, but an ontogenetic, whole‐system investigation of trigeminal tissue and associated musculature, cartilage, and bone is lacking, as is an understanding of developmental timing of morphologies significant to hypotheses of homology. Here, we use contrast‐enhanced computed tomography imaging to provide description and analysis of the trigeminal system in an ontogenetic series of Alligator mississippiensis from embryonic to adult form. We explore growth rates and allometric relationships of structures and discuss the significance to hypotheses of homology. We find a high growth rate and allometric trajectory of the trigeminal nerve in comparison to other cranial nerves, likely associated with the large volume of trigeminal musculature and high densities of sensory receptors. We identify a similar trend in the pterygoideus dorsalis muscle, the highest contributor to bite force. We narrow ontogenetic timing of features related to the trigeminal topological paradigm and the undeveloped epipterygoid. Overall, we provide a basis for understanding trigeminal development in crocodylians, which upon comparison across reptiles will reveal ontogenetic origins of morphological variation. Contrast‐enhanced computed tomography scans of the developing cranium of Alligator mississippiensis reveal morphology in four dimensions, providing a basis for understanding the developmental origins of morphological variation across reptiles.