Molecular typification of Escherichia coli from community-acquired urinary tract infections in Mexico

• Published in: International journal of antimicrobial agents 2022-10, Vol.60 (4), p.106667-106667, Article 106667
• Main Authors: Belmont-Monroy, Laura, Ribas-Aparicio, Rosa María, González-Villalobos, Edgar, Pérez-Ramos, José Alejandro, Aparicio-Ozores, Gerardo, Eslava-Campos, Carlos A., Hernández-Chiñas, Ulises, Aquino-Andrade, Alejandra, Balcázar, José Luis, Molina-López, José
• Format: Article
• Language: English
• Subjects: antibiotic resistance; community-acquired urinary tract infection; new Clermont phylotyping; uropathogenic E. coli; virulence factors
• ISSN: 0924-8579; 1872-7913
• DOI: 10.1016/j.ijantimicag.2022.106667

•E. coli O25b-ST131 clone was identified in 32% and assigned to the phylogroup B2•Some virulence genes are prevalent among the pathogenic phylogroup of UPEC strains.•Commensal phylogenetic groups are multidrug-resistant and harbored virulence genes. One hundred and five uropathogenic Escherichia coli (UPEC) strains from patients with community-acquired urinary tract infection were characterized according to their phylogenetic groups, virulence factors, serogroups, antibiotics resistance, and genotype. The pathogenic phylogenetic groups (B2, D, and F) were found in 71.4% of the tested strains. Among them, the main uropathogenic serogroups were O8, O25, and O75, in which 97.1% of the strains had a multidrug-resistant profile. Moreover, 16 virulence genes were analyzed using a combination of PCR assays, with the fimH, irp-2, iutA, aer, iucC, PAI, sat, iroN, usp, and cnf1 genes being mainly found in pathogenic phylogroups. The E. coli O25b-ST131 clone was identified in 32% of the strains assigned to the pathogenic phylogroup B2. Our findings demonstrate that virulence genes encoding adhesin components, iron acquisition systems, toxins, and pathogenicity-associated islands were highly prevalent among the pathogenic phylogroup of UPEC strains.