A designed plasmid‐transition strategy enables rapid construction of robust and versatile synthetic exoelectrogens for environmental applications

Genetic engineering is promising to expand the application scope of exoelectrogens in energy and environmental applications and plasmid vectors, as one type of fundamental tool, are intensively applied. Antibiotics are widely utilized for plasmid selection and maintenance; however, their utilization...

Full description

Saved in:
Bibliographic Details
Published in:Environmental microbiology 2022-11, Vol.24 (11), p.5292-5305
Main Authors: Fan, Yang‐Yang, Tang, Qiang, Sun, Hong, Yu, Han‐Qing
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - metabolism
Antibiotics
Cell Respiration
Chromium
Complementation
Construction
Current density
Electron transfer
Electron Transport
Gene expression
Genetic engineering
Heavy metals
Heterogeneity
Maintenance
Menaquinones
Oxidoreductions
Plasmids
Plasmids - genetics
Reduction (metal working)
Robustness
Shewanella - genetics
Shewanella - metabolism
Vectors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genetic engineering is promising to expand the application scope of exoelectrogens in energy and environmental applications and plasmid vectors, as one type of fundamental tool, are intensively applied. Antibiotics are widely utilized for plasmid selection and maintenance; however, their utilization suffers from environmental concerns on the spread of resistance genes, elevated costs, inevitable genotypic instability and phenotypic heterogeneity. In this work, we establish an auxotrophic complementation system for stable plasmid maintenance without antibiotic association, in Shewanella oneidensis, an attractive model exoelectrogen. A plasmid‐transition strategy is designed to facilitate the rapid and efficient construction of the auxotrophic complementation system. Such a system not only enables the same intensive gene expression as the conventional antibiotic‐associated plasmid system but also exhibits remarkably superior robustness and stability. With this system, the menaquinone pool of the extracellular respiratory chain is enhanced first independently and further synergized by engineering the Mtr conduit, leading to significantly promoted extracellular electron transfer (EET) outputs (up to 10.33‐ and 2.97‐fold improvement in the maximum current density and the maximum output voltage) and heavy metal Cr(VI) reduction ability (5.15‐fold improvement). This work provides a robust and stable platform to engineer exoelectrogens for environmental applications.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.16181