Metabolism and residue differences of Enrofloxacin between the brain and peripheral tissues and the resulting brain damages in crucian carp (Carassius auratus var. Pengze)

• Published in: Journal of veterinary pharmacology and therapeutics 2023-01, Vol.46 (1), p.42-51
• Main Authors: Wan, Gen, Zhang, Fan, Wang, Runping, Wei, Lili, Huang, Jianzhen, Lu, Xinmin, Cai, Zhihuan, Wang, Long, Zhong, Zhiwei, Xu, Yanyan, Ruan, Jiming
• Format: Article
• Language: English
• Subjects: brain damage; crucian carp; enrofloxacin; metabolism; residue
• ISSN: 0140-7783; 1365-2885
• DOI: 10.1111/jvp.13092

This study aimed to explore the metabolism and residue differences of Enrofloxacin (ENR) at two doses between the brain and peripheral tissues (liver, kidney, and muscle) along with the brain damages caused by ENR in crucian carp (Carassius auratus var. Pengze). The concentrations of ENR in tissues were determined using a validated high‐performance liquid chromatography (HPLC) analysis. Relying on the hematoxylin–eosin (HE) staining method, brain damages caused by the drug were evaluated by the section of pathological tissue. Metabolism and residue results showed that ENR could be detected in the brain throughout the experiment both at median lethal dose (LD50 at 96 h, 1949.84 mg/kg) and safe dose (SD, 194.98 mg/kg), as well as in the three peripheral tissues. The maximum residue at LD50 followed the decreasing order of liver >kidney > brain > muscle. Although the Cmax of ENR at SD in the brain was significantly lower than that in other peripheral tissues (p