Analysis of the A549 cell line affected by anticancer bioactive compounds of Actinomycetes isolated from saline soils

Actinomycetes are filamentous bacteria and the residents of the soil, prone to produce bioactive metabolites. This research aimed to isolate, classify, and investigate the anticancer properties of Actinomycetes secondary metabolites from various saline soils of Qom province. Actinomycetes isolates w...

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Bibliographic Details
Published in:Archives of microbiology 2022-10, Vol.204 (10), p.641-641, Article 641
Main Authors: Khoshakhlagh, Amin, Abroun, Saeid, Aghaei, Seyed Soheil, Soleimani, Mohammad, Zolfaghari, Mohammad Reza
Format: Article
Language:English
Subjects:
Actinomycetes
Anticancer properties
Antineoplastic drugs
Antitumor agents
Apoptosis
Assaying
Bacteria
BAX gene
Bcl-2 protein
Bioactive compounds
Biochemistry
Biocompatibility
Biological activity
Biomedical and Life Sciences
Biotechnology
Cancer
Cell Biology
Cytotoxicity
Drug development
Ecology
Filamentous bacteria
Flow cytometry
Gene expression
Gene sequencing
Life Sciences
Liquid chromatography
Metabolites
Microbial Ecology
Microbiology
Original Paper
Real time
Saline soils
Secondary metabolites
Soil bacteria
Soil microorganisms
Streptomyces
Toxicity
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Summary:Actinomycetes are filamentous bacteria and the residents of the soil, prone to produce bioactive metabolites. This research aimed to isolate, classify, and investigate the anticancer properties of Actinomycetes secondary metabolites from various saline soils of Qom province. Actinomycetes isolates were molecularly recognized by 16SrRNA gene sequencing after the PCR procedure. The A549 cell line was then exposed to bacterial metabolites to find their cytotoxicity by MTT assay and their capacity to cause apoptosis by Flow cytometry. The expression levels of the bax and bcl-2 genes were determined using Real-time PCR. Bacterial metabolites were distinct by HPLC and GC–MS assays. Sequencing identified three novel Actinomycetes strains, Streptomyces griseoflavus , Streptomyces calvus , and Kitasatospora phosalacineus . The IC 50 doses of bacterial metabolites were discovered equal to 1337, 2619, and 4874 µg/ml, respectively. Flow cytometric assay revealed that their secondary metabolites were capable of inducing apoptosis in A549 cells by 25%, 14.5%, and 7.58%, respectively. Real-time PCR findings displayed that the bax gene expression in A549 cells treated with S. griseoflavus and S. calvus , comparatively increased ( P  
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-03258-1