Experimental Clarification of PRPS-1 Structural Essentials

Phosphoribosyl pyrophosphate synthetase-1 (PRPS-1; EC 2.7.6.1.) catalyzes the binding of phosphate-group to ribose 5-phosphate, forming the 5-phosphoribosyl-1-pyrophosphate, which is necessary for the salvage pathways of purine and pyrimidine, pyridine nucleotide cofactors - NAD and NADP, the amino...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2022-12, Vol.80 (4), p.699-709
Main Authors: Danielyan, Kristine E., Grabski, Hovakim V., Ginosyan, Siranuysh V., Chailyan, Samvel G., Tiratsuyan, Susanna G.
Format: Article
Language:English
Subjects:
Amino acids
Antibiotics
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biosynthesis
Biotechnology
Cell Biology
Cofactors
Conserved sequence
Effectors
Enzymes
Histidine
Life Sciences
Molecular docking
Nucleotides
Original Paper
Pharmacology/Toxicology
Phosphates
Phosphoribosyl pyrophosphate
Ribose
Tryptophan
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Summary:Phosphoribosyl pyrophosphate synthetase-1 (PRPS-1; EC 2.7.6.1.) catalyzes the binding of phosphate-group to ribose 5-phosphate, forming the 5-phosphoribosyl-1-pyrophosphate, which is necessary for the salvage pathways of purine and pyrimidine, pyridine nucleotide cofactors - NAD and NADP, the amino acids histidine and tryptophan biosynthesis. We aimed to investigate the impact of the different effectors on the activity of PRPS-1, to check the activity of the enzyme in vitro in a wide range of pHs and investigate some structural essentials of the enzyme, isolated from brain and liver. Molecular docking analyses were used to delineate the essentials of PRPS-1 structure, to find out the existence of enzyme effectors. Previously created by us kit was used for determination of the activity of PRPS-1 based on the formation of the inorganic phosphates (λ = 700 nm, Cary 60, Agilent, USA). Effectors impact on the activity of PRPS-1 was evaluated. In silico results of the effectors were later proven by in vitro experiments. For the first time biochemical essentials, including the existence of the multiple pockets, involvement of the amino acids into the processes of interactions with the effectors, evolutional of the sequence conservation, tissue depended V max differences were identified.
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-022-01104-1