Evaluating the in vitro digestion of lipids rich in medium-chain fatty acids (MCFAs) using dynamic and static protocols

•The dynamic GI model was valuable to simulate and predict lipolysis rates;•Gastric lipase activity limited the extent of lipolysis during gastric digestion;•The dynamic protocol delivery of simulated gastric fluid regulated intragastric pH;•Free fatty acid profiles and concentrations related direct...

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Bibliographic Details
Published in:Food chemistry 2023-04, Vol.406, p.135080-135080, Article 135080
Main Authors: Pereira, Ericsem, Fernandes, Jean-Michel, Gonçalves, Raquel, Pinheiro, Ana C., Salomé Duarte, M., Madalena Alves, M., Meirelles, Antonio J.A., Maximo, Guilherme J., Vicente, Antonio A.
Format: Article
Language:English
Subjects:
carboxylic ester hydrolases
Coconut oil
Digestion
digestive tract
dodecanoic acid
esterification
Fatty Acids - analysis
food chemistry
Gastrointestinal models
Hydrolysis
ileum
INFOGEST protocol
jejunum
Lipid digestion
Lipolysis
Medium-chain triglycerides (MCT)
Stomach - chemistry
Triglycerides
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Summary:•The dynamic GI model was valuable to simulate and predict lipolysis rates;•Gastric lipase activity limited the extent of lipolysis during gastric digestion;•The dynamic protocol delivery of simulated gastric fluid regulated intragastric pH;•Free fatty acid profiles and concentrations related directly to lipase activities. Investigating the digestion of lipids is paramount for developing new lipid-based products. This work evaluated the gastrointestinal (GI) digestion of medium-chain fatty acids (MCFAs) rich lipids. The dynamic GI in vitro system was used to simulate gastric, duodenal, jejunal, and ileal GI tract portions. Results from the dynamic protocol were compared against static in vitro assays and GC analyses were conducted to assess the FA profile of FFA released during digestion. Caprylic and capric acids released during the gastric digestion of MCT oil varied from 61–63% and 36–38% of total esterified FA, respectively. Lauric acid was the most representative FFA released (31–54%) during the gastric digestion of coconut oil samples. It was observed that the gastric digestion phase plays a crucial role in the MCFA lipolysis and the lipase activity restricted the amount of free MCFA liberated during the GI digestion, resulting in incomplete lipids hydrolysis.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2022.135080