Recognition of Core-Fucosylated Glycopeptides Based on the Y1+Fuc/Y1 Ratio in Low-Energy HCD Spectra

Accurate identification of core fucosylation on N-glycopeptides remains challenging due to fucose migration during mass spectrometry analysis. Here, we introduce a simple and straightforward method for core-fucosylated glycopeptide recognition based on the relative intensities of Y1+Fuc ions compare...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-12, Vol.94 (50), p.17349-17353
Main Authors: Chen, Zexuan, Shen, Jiechen, Dong, Wenbo, Li, Pengfei, Xin, Miaomiao, Liu, Didi, Jia, Li, Zhu, Bojing, Li, Wenzhe, Sun, Shisheng
Format: Article
Language:English
Subjects:
Animals
Antennas
Antibiotics
Brain
Chemistry
Energy
Fucose
Fucose - chemistry
Glycopeptides
Glycopeptides - analysis
Glycoproteins
Glycoproteins - chemistry
Glycosylation
Ions
Mass spectrometry
Mass spectroscopy
Mice
Recognition
Spectra
Tandem Mass Spectrometry - methods
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Summary:Accurate identification of core fucosylation on N-glycopeptides remains challenging due to fucose migration during mass spectrometry analysis. Here, we introduce a simple and straightforward method for core-fucosylated glycopeptide recognition based on the relative intensities of Y1+Fuc ions compared with their corresponding Y1 ions (labeled as Y1+Fuc/Y1 or simply Y1F/Y1 ratio > 0.1) in low-energy HCD-based spectra. The method was first developed by systematically evaluating the influence of fucose migration on the Y1F ion from antenna fucoses based on the distribution of the Y1F/Y1 ratios in the MS/MS spectra of antenna-fucosylated glycopeptides from Fut8–/– mouse brain. The feasibility of the method was then confirmed by using two standard glycoproteins, comparison with glycopeptides in Fut8+/+ mouse brain with/without in silico core-fucosylation removal, and Y1F/Y1 ratio alterations under a lower HCD energy. This method will be applicable to the manual interpretation and software-based high-throughput analysis of core-fucosylated glycopeptides.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c03182