Substrate-Dependent Conformational Switch of the Noncubane [4Fe-4S] Cluster in Heterodisulfide Reductase HdrB

• Published in: Journal of the American Chemical Society 2023-01, Vol.145 (1), p.7-11
• Main Authors: Pelmenschikov, Vladimir, Ferreira, Delfim, Venceslau, Sofia S., Hildebrandt, Peter, Pereira, Inês A. C., Todorovic, Smilja
• Format: Article
• Language: English
• Subjects: Electron Spin Resonance Spectroscopy; Iron - chemistry; Iron-Sulfur Proteins - chemistry; Oxidoreductases - metabolism; Spectrum Analysis, Raman
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/jacs.2c10885

The noncubane [4Fe-4S] cluster identified in the active site of heterodisulfide reductase (HdrB) displays a unique geometry among Fe–S cofactors found in metalloproteins. Here we employ resonance Raman (RR) spectroscopy and density functional theory (DFT) calculations to probe structural, electronic, and vibrational properties of the noncubane cluster in HdrB from a non-methanogenic Desulfovibrio vulgaris (Dv) Hildenborough organism. The immediate protein environment of the two neighboring clusters in DvHdrB is predicted using homology modeling. We demonstrate that in the absence of substrate, the oxidized [4Fe-4S]3+ cluster adopts a “closed” conformation. Upon substrate coordination at the “special” iron center, the cluster core translates to an “open” structure, facilitated by the “supernumerary” cysteine ligand switch from iron-bridging to iron-terminal mode. The observed RR fingerprint of the noncubane cluster, supported by Fe–S vibrational mode analysis, will advance future studies of enzymes containing this unusual cofactor.