A One-Step Multiplex PCR Method to Rapidly Distinguish Two Strains of Diglyphus wani (Hymenoptera: Eulophidae) Against Agromyzid Leafminers

Hymenopteran parasitoids generally show a haplo-diploid sex determination system. Haploid males are produced from unfertilized eggs, whereas diploid females develop from fertilized eggs (arrhenotokous). In some cases, diploid females develop from unfertilized eggs (thelytokous). Diglyphus wani (Hyme...

Full description

Saved in:
Bibliographic Details
Published in:Journal of economic entomology 2023-02, Vol.116 (1), p.256-262
Main Authors: Du, Su-Jie, Xu, Shi-Yun, Guo, Jian-Yang, Ye, Fu-Yu, Wan, Wei-Jie, Liu, Wan-Xue
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological control
Biological Control Agents
Cytochrome
Cytochrome oxidase
Cytochrome-c oxidase
Design
Developmental stages
Diploids
Eggs
Entomology
Eulophidae
Female
Females
Hymenoptera
Hymenoptera - genetics
Identification
Laboratories
Male
Males
Methods
mitochondrial gene cytochrome c oxidase I
MOLECULAR ENTOMOLOGY
molecular identification
Morphology
Multiplex Polymerase Chain Reaction
Physical characteristics
Polymerase chain reaction
rapid method
Sex determination
Wasps
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hymenopteran parasitoids generally show a haplo-diploid sex determination system. Haploid males are produced from unfertilized eggs, whereas diploid females develop from fertilized eggs (arrhenotokous). In some cases, diploid females develop from unfertilized eggs (thelytokous). Diglyphus wani (Hymenoptera: Eulophidae) is a biological control agent for agromyzid leafminers and have arrhenotokous and thelytokous strains. However, the morphological characteristics of two strains of D. wani are so similar that it is difficult to accurately distinguish them based on morphology. Here, a rapid molecular identification method was developed based on the mitochondrial gene cytochrome c oxidase I (COI) and one-step multiplex PCR. Two primer combinations, PC1 (Ar-F1/Th-F1/WR2) and PC2 (Ar-F1/Th-F4/WR2), were designed and repeatedly screened to distinguish two strains simultaneously, of which two special forward primers Th-F1/Th-F4 were used for the thelytokous strain and one special forward primer Ar-F1 was used for the arrhenotokous strain. In addition, a common reverse primer, WR2, was used for both strains. The PC1 and PC2 PCR assays were effective in distinguishing the two strains at different developmental stages and field colonies. This method provides a reliable, highly sensitive, and cost-effective tool for the rapid identification of the two strains of D. wani.
ISSN:0022-0493
1938-291X
DOI:10.1093/jee/toac197