Genome-wide identification and expression analysis of metal tolerance protein (MTP) gene family in soybean (Glycine max) under heavy metal stress

• Published in: Molecular biology reports 2023-04, Vol.50 (4), p.2975-2990
• Main Authors: El-Sappah, Ahmed H., Abbas, Manzar, Rather, Shabir A., Wani, Shabir H., Soaud, Nourhan, Noor, Zarqa, Qiulan, Huang, Eldomiaty, Ahmed S., Mir, Reyazul Rouf, Li, Jia
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Gene expression; Gene Expression Regulation, Plant - genetics; Genomes; Glycine max - genetics; Glycine max - metabolism; Heavy metals; Histology; Leaves; Life Sciences; Manganese; Metal ions; Metals, Heavy - metabolism; Metals, Heavy - toxicity; Morphology; Original Article; Phylogeny; Plant Proteins - metabolism; Progress in genomics; Soybeans; Stress, Physiological - genetics; transcriptomics and breeding of crops
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-022-08100-x

Aim Plant metal tolerance proteins (MTPs) are plant membrane divalent cation transporters that specifically contribute to heavy metal stress resistance and mineral uptake. However, little is known about this family’s molecular behaviors and biological activities in soybean. Methods and results A total of 20 potential MTP candidate genes were identified and studied in the soybean genome for phylogenetic relationships, chromosomal distributions, gene structures, gene ontology, cis-elements, and previous gene expression. Furthermore, the expression of MTPs has been investigated under different heavy metals treatments. All identified soybean MTPs ( GmaMTP s) contain a cation efflux domain or a ZT dimer and are further divided into three primary cation diffusion facilitator (CDF) groups: Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. The developmental analysis reveals that segmental duplication contributes to the GmaMTP family’s expansion. Tissue-specific expression profiling revealed comparative expression profiling in similar groups, although gene expression differed between groups. GmaMTP genes displayed biased responses in either plant leaves or roots when treated with heavy metal. In the leaves and roots, nine and ten GmaMTP s responded to at least one metal ion treatment. Furthermore, in most heavy metal treatments, GmaMTP1.1 , GmaMTP1.2 , GmaMTP3.1 , GmaMTP3.2 , GmaMTP4.1 , and GmaMTP4.3 exhibited significant expression responses. Conclusion Our findings provided insight into the evolution of MTPs in soybean. Overall, our findings shed light on the evolution of the MTP gene family in soybean and pave the path for further functional characterization of this gene family.