The anti-infective potential of the endophytic fungi associated with Allium cepa supported by metabolomics analysis and docking studies

Endophytic fungi are known to be a rich source of anti-infective drugs. In our study, was investigated for fungal diversity using different media to give 11 isolates which were identified morphologically. Out of the isolated fungal strains, sp. (LCEF10) revealed potential anti-infective activity aga...

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Published in:Natural product research 2023-12, Vol.37 (23), p.1-4068
Main Authors: Hassan, Hind Ashraf, Abdelhafez, Omnia Hesham, Shady, Nourhan Hisham, Yahia, Ramadan, Mohamed, Nada M, Rateb, Mostafa E, Akil, Lina, Khadra, Ibrahim, Glaeser, Stefanie P, Kämpfer, Peter, Abdelmohsen, Usama Ramadan, El-Katatny, Mo'men Hamed
Format: Article
Language:English
Subjects:
Acetic acid
Allium cepa
Antiinfectives and antibacterials
Endophytes
Ethyl acetate
Fungi
Metabolomics
Molecular docking
Penicillium
Phenols
Phylogeny
Polyketides
Terpenes
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Summary:Endophytic fungi are known to be a rich source of anti-infective drugs. In our study, was investigated for fungal diversity using different media to give 11 isolates which were identified morphologically. Out of the isolated fungal strains, sp. (LCEF10) revealed potential anti-infective activity against the tested microbes ( ATTC 25922, aeruginosa (ATTC 29231), ATTC 27853 ATTC 10231), besides, their MICs were measured by well diffusion method, therefore, it was subjected to molecular identification in addition to phylogenetic analysis. Moreover, the ITS sequence of strain LCEF10 showed a consistent assignment with the highest sequence similarity (99.81%) to NRRL 787. The crude ethyl acetate extract of sp. LCEF10 was investigated for metabolomic analysis using LC-HR-ESI-MS. The metabolic profiling revealed the presence of polyketides, macrolides, phenolics and terpenoids. Furthermore, molecular docking study was carried out to predict which compounds most likely responsible for the anti-infective activity.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2023.2167204