Stereoselective analysis of chiral succinate dehydrogenase inhibitors (SDHIs) in foods of plant origin and animal origin by supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS)

• Published in: Food chemistry 2023-06, Vol.411, p.135452-135452, Article 135452
• Main Authors: Di, Shanshan, Zhao, Huiyu, Liu, Zhenzhen, Lu, Yuele, Xu, Hao, Qi, Peipei, Wang, Zhiwei, Wang, Xinquan
• Format: Article
• Language: English
• Subjects: Absolute configuration; Animals; Chiral SDHIs; Chromatography, High Pressure Liquid; Chromatography, Supercritical Fluid - methods; Enantioseparation; Food; Quantitative analysis; Stereoisomer preparation; Stereoisomerism; Succinate Dehydrogenase; Tandem Mass Spectrometry - methods
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2023.135452

[Display omitted] •The methods of stereoisomer preparation of chiral SDHIs were established.•The absolute configuration of chiral SDHI stereoisomers were confirmed.•Enantioseparation methods of chiral SDHIs were established through SFC-MS/MS.•An integrated QuEChERS strategy was used to analyse chiral SDHIs in foods. The stereoisomers of chiral SDHIs were prepared using Autoprep HPLC and chiral columns. The method of combining theoretical calculation with experimental determination was used to confirm the absolute configuration of stereoisomer. SFC-MS/MS and four kinds of chiral columns were used to separate the eight chiral SDHIs, and they could be separated simultaneously using OD-3 column in 6.5 min. The integrated QuEChERS strategy was used to analyse the chiral SDHIs in foods of plant and animal origin, and the average recoveries ranged from 71 % to 119 % with RSD ≤ 18 %, and the LOQ was 1 ng/g. There were 99.2 % and 63.6 % matrix effects were in the range of 0.8–1.2 in foods of plant and animal origin, respectively, showing weak matrix effects. The study provided methods for monitoring chiral SDHIs stereoisomers residues, which were crucial for stereoselective evaluations and improving risk assessments.