Plasma riboflavin fluorescence as a diagnostic marker of mesenteric ischemia-reperfusion injury in rats

Due to the delayed and vague symptoms, it is difficult to early diagnose mesenteric ischemia injuries in the dynamics of acute illness, leading to a 60–80 % mortality rate. Here, we found plasma fluorescence spectra can rapidly assess the severity of mesenteric ischemia injury in animal models. Isch...

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Bibliographic Details
Published in:Thrombosis research 2023-03, Vol.223, p.146-154
Main Authors: Wu, Xueqin, Guo, Lun-Zhang, Liu, Yi-Hung, Liu, Yu-Cheng, Yang, Po-Lun, Leung, Yun-Shiuan, Tai, Hwan-Ching, Wang, Tzung-Dau, Lin, Jesse Chih-Wei, Lai, Chao-Lun, Chuang, Yueh-Hsun, Lin, Chih-Hsueh, Chou, Pi-Tai, Lai, I-Rue, Liu, Tzu-Ming
Format: Article
Language:English
Subjects:
Animals
Disease Models, Animal
Humans
Mesenteric Ischemia
Plasma fluorophores
Rats
Rats, Wistar
Reperfusion Injury
Riboflavin
Ultrahigh performance liquid chromatography
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Summary:Due to the delayed and vague symptoms, it is difficult to early diagnose mesenteric ischemia injuries in the dynamics of acute illness, leading to a 60–80 % mortality rate. Here, we found plasma fluorescence spectra can rapidly assess the severity of mesenteric ischemia injury in animal models. Ischemia-reperfusion damage of the intestine leads to multiple times increase in NADH, flavins, and porphyrin auto-fluorescence of blood. The fluorescence intensity ratio between blue-fluorophores and flavins can reflect the occurrence of shock. Using liquid chromatography and mass spectroscopy, we confirm that riboflavin is primarily responsible for the increased flavin fluorescence. Since humans absorb riboflavin from the intestine, its increase in plasma may indicate intestinal mucosa injury. Our work suggests a self-calibrated and reagent-free approach to identifying the emergence of fatal mesenteric ischemia in emergency departments or intensive care units.
ISSN:0049-3848
1879-2472
DOI:10.1016/j.thromres.2023.01.032