Polygalacturonase treatment affects carotenoid absorption from veggie juice

• Published in: Food chemistry 2023-07, Vol.415, p.135748-135748, Article 135748
• Main Authors: Liu, Jianing, Bi, Jinfeng, Liu, Xuan, Liu, Dazhi, Lyu, Jian, Liu, Meng, Verkerk, Ruud, Dekker, Matthijs, Fogliano, Vincenzo
• Format: Article
• Language: English
• Subjects: absorption; beta Carotene - chemistry; bioavailability; Biological Availability; Caco-2 Cells; Carotenoid absorption; Carotenoid bioaccessibility; Carotenoids - chemistry; Carotenoids - metabolism; carrot juice; digestion; Emulsion; emulsions; food chemistry; Fruit and Vegetable Juices; Humans; lipophilicity; lutein; Lutein - chemistry; particle size; peaches; Pectin; polygalacturonase; Polygalacturonase - chemistry; Polygalacturonase - metabolism; Polygalacturonase - pharmacology; viscosity; zeaxanthin; Zeaxanthins - chemistry
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2023.135748

•Polygalacturonase treatment decreased particle size and viscosity of veggie juice.•Polygalacturonase treatment improved carotenoid bioaccessibility of veggie juice.•Xanthophylls had higher cellular uptake and transport ratios than carotenes.•Factors that improve bioaccessibility did not necessarily affect the absorption. The present study was conducted to investigate the effects of polygalacturonase (PG) treatment on carotenoid absorption upon digestion of HPH-treated combined peach and carrot juice (CJ) with or without the presence of lipids. Results showed that PG treatment reduced median particle diameter (D50) and viscosity of CJ, and increased total carotenoid bioaccessibility by 41%. In the presence of emulsion, the bioaccessibility of carotenoids was higher and it was not significantly affected by PG treatment. Xanthophylls (lutein and zeaxanthin) had higher bioaccessibility than the more lipophilic carotenes (β-carotene and α-carotene); also, uptake in Caco-2 cells and transport of lutein and zeaxanthin were higher than for β-carotene and α-carotene. Individual carotenoids bioaccessibility was negatively correlated with their transport. All together data showed digestion and absorption processes were two independent processes: factors improving carotenoid bioaccessibility did not necessarily affect their bioavailability.